Recombinant
RabMAb

Recombinant Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (ab192261)

Overview

  • Product name

    Anti-CRTAP/CASP antibody [EPR15121] - N-terminal
    See all CRTAP/CASP primary antibodies
  • Description

    Rabbit monoclonal [EPR15121] to CRTAP/CASP - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human CRTAP/CASP aa 1-100. The exact sequence is proprietary.
    Database link: O75718

  • Positive control

    • Saos-2, SW1353 and HeLa cell lysate. Human endometrium tissue. Human adenocarcinoma of colon tissue.
  • General notes

     

     

     This product was previously labelled as CRTAP

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 47 kDa.

Target

  • Relevance

    CRTAP/CASP is found in articular chondrocytes and is expressed in a variety of other tissues. Defects in CRTAP/CASP are the cause of osteogenesis imperfecta type 7 (OI-7). OI is a connective tissue disorder characterized by bone fragility and low bone mass. OI-7 is an autosomal recessive form of OI.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Cartilage associated protein antibody
    • CASP antibody
    • Leprecan like 3 antibody
    • LEPREL3 antibody
    • OI7 antibody
    see all

Images

  • All lanes : Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (ab192261) at 1/1000 dilution

    Lane 1 : Saos-2 cell lysate
    Lane 2 : SW1353 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin embedded human endometrial tissue sections labeling CRTAP/CASP using ab192261 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue sections labeling CRTAP/CASP using ab192261 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab192261 has not yet been referenced specifically in any publications.

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