Recombinant
RabMAb

Recombinant Anti-CRTC3 antibody [EPR3440] - BSA and Azide free (ab220809)

Overview

  • Product name

    Anti-CRTC3 antibody [EPR3440] - BSA and Azide free
    See all CRTC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3440] to CRTC3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRTC3. The exact sequence is proprietary.

  • Positive control

    • 293T and Jurkat cell lysates and Human tonsil tissue
  • General notes

    Ab220809 is the carrier-free version of ab91654. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab220809 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab220809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 67 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23033494
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
    • Tissue specificity

      Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
    • Sequence similarities

      Belongs to the TORC family.
    • Cellular localization

      Nucleus. Cytoplasm. Appears to be mainly nuclear.
    • Information by UniProt
    • Database links

    • Alternative names

      • CREB regulated transcription coactivator 3 antibody
      • CREB-regulated transcription coactivator 3 antibody
      • CRTC 3 antibody
      • CRTC3 antibody
      • CRTC3_HUMAN antibody
      • FLJ21868 antibody
      • TORC 3 antibody
      • TORC-3 antibody
      • TORC3 antibody
      • Transducer of CREB protein 3 antibody
      • Transducer of regulated cAMP response element binding protein (CREB) 3 antibody
      • Transducer of regulated cAMP response element binding protein 3 antibody
      • Transducer of regulated cAMP response element-binding protein 3 antibody
      • Transducer of regulated CREB protein 3 antibody
      see all

    Images

    • Immunofluorescence staining of Jurkat cells with purified ab91654 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab91654 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91654).

    • Immunohistochemical staining of paraffin embedded rat spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91654).

    • Immunohistochemical staining of paraffin embedded mouse spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91654).

    • Immunohistochemical staining of paraffin embedded human tonsil with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91654).

    • ChIP analysis using ab91654 binding CRTC3 in Human neuroblastoma cells. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (0.2µg/10^6 cells) for 12 hours at 4°C. Protein binding was detected using real-time PCR.
      Positive control: GAPDH.
      Negative Control: Primers for region not expressed.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91654).

    • Unpurified ab91654 at 1/100 dilution staining CRTC3 in Human tonsil by immunohistochemistry (paraffin-embedded section).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91654).

    References

    This product has been referenced in:

    • Jurek B  et al. Oxytocin Regulates Stress-Induced Crf Gene Transcription through CREB-Regulated Transcription Coactivator 3. J Neurosci 35:12248-60 (2015). WB, ChIP ; Human . Read more (PubMed: 26338335) »
    • Patel K  et al. The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver. Nat Commun 5:4535 (2014). WB . Read more (PubMed: 25088745) »
    See all 4 Publications for this product

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