Overview

  • Product name
  • Description
    Rabbit polyclonal to CRTR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CRTR1 aa 221-251 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9NZ16

  • Positive control
    • WB: WiDr cell lysate and mouse stomach tissue lysate. IHC-P: Human stomach tissue. Flow Cyt: WiDr cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab171839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Transcriptional suppressor. May suppress UBP1-mediated transcriptional activation. Modulates the placental expression of CYP11A1.
  • Tissue specificity
    Highly expressed in placental JEG-3 cells and very low levels of expression in non-steroidogenic cells. No expression was seen in adrenal NCI-H295A cells or in adrenal tissue.
  • Sequence similarities
    Belongs to the grh/CP2 family. CP2 subfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP2 related transcriptional repressor 1 antibody
    • CP2-related transcriptional repressor 1 antibody
    • CRTR 1 antibody
    • CRTR-1 antibody
    • LBP 9 antibody
    • LBP9 antibody
    • TF2L1_HUMAN antibody
    • TFCP2 L1 antibody
    • TFCP2L1 antibody
    • Transcription factor CP2 like 1 antibody
    • Transcription factor CP2 like protein 1 antibody
    • Transcription factor CP2-like protein 1 antibody
    • Transcription factor LBP 9 antibody
    • Transcription factor LBP-9 antibody
    • Transcription factor LBP9 antibody
    see all

Images

  • Anti-CRTR1 antibody (ab171839) + Mouse stomach tissue lysates at 35 µg

    Predicted band size: 55 kDa

  • Anti-CRTR1 antibody (ab171839) + WiDr cell line lysate at 35 µg

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labeling CRTR1 with ab171839. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram) labeling CRTR1 with ab171839 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab171839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (CJ7 Embryonic Stem cells)
Permeabilization
Yes - Triton-X100
Specification
CJ7 Embryonic Stem cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde

Mr. Dylan Stavish

Verified customer

Submitted Jun 13 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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