Key features and details
- Rabbit polyclonal to CRY2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CRY2 antibody
See all CRY2 primary antibodies
DescriptionRabbit polyclonal to CRY2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 543-593 of Human CRY2 (AAH41814.1)
- Whole cell lysate from HeLa and 293T cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 67 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBlue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK
NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK
NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.
Tissue specificityExpressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.
Sequence similaritiesBelongs to the DNA photolyase class-1 family.
Contains 1 DNA photolyase domain.
modificationsPhosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.
Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation.
Cellular localizationCytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
- Information by UniProt
- cry2 antibody
- CRY2_HUMAN antibody
- cryptochrome 2 (photolyase like) antibody
All lanes : Anti-CRY2 antibody (ab93802) at 0.04 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Lane 4 : whole 293T cell lysate at 50 µg
Predicted band size: 67 kDa
Immunoprecipitation analysis of CRY2 expression in whole HeLa cells lysate (1 mg for IP, 20% of IP loaded) using ab93802 at 3 ug/mg lysate (Left Lane). Right lane represent IP antibody control. Subsequent WB analysis was performed using 1ug/ml ab93802.
ab93802 has been referenced in 1 publication.
- Gorelik M et al. A Structure-Based Strategy for Engineering Selective Ubiquitin Variant Inhibitors of Skp1-Cul1-F-Box Ubiquitin Ligases. Structure 26:1226-1236.e3 (2018). PubMed: 30033217