Overview

  • Product name

  • Description

    Rabbit polyclonal to CRYBA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Pig, Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 - 150 of Human CRYBA4 (NP_001877).

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lyates IF: HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab97771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Crystallins are the dominant structural components of the vertebrate eye lens.
  • Involvement in disease

    Defects in CRYBA4 are the cause of cataract zonular type 2 (CZ2) [MIM:610425]; also known as lamellar cataract 2. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
    Defects in CRYBA4 are a cause of microphthalmia isolated with cataract type 4 (MCOPCT4) [MIM:610426]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
  • Sequence similarities

    Belongs to the beta/gamma-crystallin family.
    Contains 4 beta/gamma crystallin 'Greek key' domains.
  • Domain

    Has a two-domain beta-structure, folded into four very similar Greek key motifs.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta A4 crystallin antibody
    • Beta crystallin A4 antibody
    • Beta-A4 crystallin antibody
    • Beta-crystallin A4 antibody
    • CRBA4_HUMAN antibody
    • CRYBA4 antibody
    • Crystallin beta A4 antibody
    • Crystallin, beta polypeptide A4 antibody
    • CTRCT23 antibody
    • Eye lens structural protein antibody
    • MCOPCT4 antibody
    see all

Images

  • All lanes : Anti-CRYBA4 antibody (ab97771) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa



    12% SDS PAGE
  • ab97771, at a 1/200 dilution, staining CRYBA4 in paraformaldehyde-fixed HeLa by Immunofluorescence analysis. Right image is merged with a DNA probe.

References

ab97771 has not yet been referenced specifically in any publications.

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