Key features and details
- Rabbit polyclonal to CRYBA4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CRYBA4 antibody
See all CRYBA4 primary antibodies
DescriptionRabbit polyclonal to CRYBA4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Pig, Monkey
Recombinant protein fragment corresponding to a region within amino acids 1 - 150 of Human CRYBA4 (NP_001877).
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lyates IF: HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 22 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionCrystallins are the dominant structural components of the vertebrate eye lens.
Involvement in diseaseDefects in CRYBA4 are the cause of cataract zonular type 2 (CZ2) [MIM:610425]; also known as lamellar cataract 2. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
Defects in CRYBA4 are a cause of microphthalmia isolated with cataract type 4 (MCOPCT4) [MIM:610426]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
Sequence similaritiesBelongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains.
DomainHas a two-domain beta-structure, folded into four very similar Greek key motifs.
- Information by UniProt
- Beta A4 crystallin antibody
- Beta crystallin A4 antibody
- Beta-A4 crystallin antibody
All lanes : Anti-CRYBA4 antibody (ab97771) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE
ab97771, at a 1/200 dilution, staining CRYBA4 in paraformaldehyde-fixed HeLa by Immunofluorescence analysis. Right image is merged with a DNA probe.
ab97771 has not yet been referenced specifically in any publications.