• Product name

    Anti-Cryptochrome I/CRY1 antibody
    See all Cryptochrome I/CRY1 primary antibodies
  • Description

    Rabbit polyclonal to Cryptochrome I/CRY1
  • Host species

  • Specificity

    Detects recombinant human Cryptochrome 1 (Cry 1) (not tested on endogenous protein yet).
  • Tested applications

    Suitable for: ICC, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Synthetic peptide corresponding to Mouse Cryptochrome I/CRY1 aa 594-606.


    (Peptide available as ab5005)

  • Positive control

    • recombinant protein
  • General notes

     This product was previously labelled as Cryptochrome I




Our Abpromise guarantee covers the use of ab3518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).


  • Function

    Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK
    ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. CRY1 and CRY2 have redundant functions but also differential and selective contributions at least in defining the pace of the SCN circadian clock and its circadian transcriptional outputs. More potent transcriptional repressor in cerebellum and liver than CRY2, though more effective in lengthening the period of the SCN oscillator. On its side, CRY2 seems to play a critical role in tuning SCN circadian period by opposing the action of CRY1. With CRY2, is dispensable for circadian rhythm generation but necessary for the development of intercellular networks for rhythm synchrony. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. Interacts with CLOCK-ARNTL/BMAL1 independently of PER proteins and is found at CLOCK-ARNTL/BMAL1-bound sites, suggesting that CRY may act as a molecular gatekeeper to maintain CLOCK-ARNTL/BMAL1 in a poised and repressed state until the proper time for transcriptional activation. Represses the CLOCK-ARNTL/BMAL1 induced transcription of BHLHE40/DEC1. Represses the CLOCK-ARNTL/BMAL1 induced transcription of ATF4, MTA1, KLF10 and NAMPT (By similarity). May repress circadian target genes expression in collaboration with HDAC1 and HDAC2 through histone deacetylation. Mediates the clock-control activation of ATR and modulates ATR-mediated DNA damage checkpoint. In liver, mediates circadian regulation of cAMP signaling and gluconeogenesis by binding to membrane-coupled G proteins and blocking glucagon-mediated increases in intracellular cAMP concentrations and CREB1 phosphorylation. Besides its role in the maintenance of the circadian clock, is also involved in the regulation of other processes. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by binding to glucocorticoid response elements (GREs). Plays a key role in glucose and lipid metabolism modulation, in part, through the transcriptional regulation of genes involved in these pathways, such as LEP or ACSL4.
  • Sequence similarities

    Belongs to the DNA photolyase class-1 family.
    Contains 1 photolyase/cryptochrome alpha/beta domain.
  • Post-translational

    Phosphorylation on Ser-247 by MAPK is important for the inhibition of CLOCK-ARNTL/BMAL1-mediated transcriptional activity. Phosphorylation by CSNK1E requires interaction with PER1 or PER2. Phosphorylation at Ser-71 and Ser-280 by AMPK decreases protein stability. Phosphorylation at Ser-568 exhibits a robust circadian rhythm with a peak at CT8, increases protein stability, prevents SCF(FBXL3)-mediated degradation and is antagonized by interaction with PRKDC.
    Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complexes, regulating the balance between degradation and stabilization. The SCF(FBXL3) complex is mainly nuclear and mediates ubiquitination and subsequent degradation of CRY1. In contrast, cytoplasmic SCF(FBXL21) complex-mediated ubiquitination leads to stabilize CRY1 and counteract the activity of the SCF(FBXL3) complex. The SCF(FBXL3) and SCF(FBXL21) complexes probably mediate ubiquitination at different Lys residues. Ubiquitination at Lys-11 and Lys-107 are specifically ubiquitinated by the SCF(FBXL21) complex but not by the SCF(FBXL3) complex. Ubiquitination may be inhibited by PER2.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other clock proteins such as PER2 or ARNTL/BMAL1.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cry1 antibody
    • CRY1_HUMAN antibody
    • Cryptochrome 1 (photolyase like) antibody
    • Cryptochrome 1 antibody
    • Cryptochrome-1 antibody
    • PHLL1 antibody
    • Photolyase 1 antibody
    • Photolyase-like antibody
    see all


This product has been referenced in:

  • Liu L  et al. Over-expressed microRNA-181a reduces glomerular sclerosis and renal tubular epithelial injury in rats with chronic kidney disease via down-regulation of the TLR/NF-?B pathway by binding to CRY1. Mol Med 24:49 (2018). Read more (PubMed: 30241461) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Rat Tissue lysate - whole (Brain (nucleus accumbens))
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
3.5 µg
Brain (nucleus accumbens)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 11 2017


I am very sorry to hear that you are still having difficulties using this antibody. I have been informed that we cannot supply the recombinant protein positive control, however good positive controls for Cryptochrome 1 are human hepatocarcinoma and human placental tissue lysate. The laboratory also wished to highlight the following: "Please note that the "correct size" (~70 kDa) is for the detection of full length recombinant human Cry1 protein, and the endogenous protein may be detected at a different size due to any number of post translational modifications. Incubating with the neutralizing peptide will reveal exactly which bands are non-specific, and which band(s) represent the protein of interest." I would be happy to arrange for the neutralizing peptide to be added to our catalogue as soon as possible if you would be interested, please just let me know. I hope that this information is helpful, please let me know if you have any additional questions.

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I'm very sorry to hear you are still experiencing problems with ab3518. This antibody has not been tested against the endogenous form of the protein so we do not know what optimal positive control lysate should be used, however a brief litterature search suggests that chryptochrome 1 should be highly expressed in the inner retina. Regarding its levels in the brain, it seems that levels can be very variable, and this may be why you do not see a band for chryptochrome 1 in your lysates. A Sancar states in the reference" Regulation of the Mammalian Circadian Clock by Cryptochrome", J. Biol. Chem., Vol. 279, Issue 33, 34079-34082, August 13, 2004 : "In the brain, mCry1 was highly expressed in the SCN, and expression exhibited a daily oscillation, peaking at about 2:00 p.m. and reaching its nadir at around 2:00 a.m.". I would like to also make the following suggestions. 1. The secondary antibody might be binding additional proteins. Make certain to run a secondary-only control. This gives an idea if the non-specific bands are due to interaction with the secondary antibody. 2. The concentration of the primary antibody used might be excessive. Decrease the amount of primary antibody that is being used (try a 1:2000 dilution for WB). Also, decrease the primary incubation period. 3. The large amount of protein that is added may be leading to non-specific banding. We generally recommend loading 10-30 ug of protein. 4. The addition of a detergent (i.e. Tween-20) may also help in reducing non-specific bands. 5. Increase the number of washes. If the wash was performed for 30 minutes changing every 5 minutes, then increase salt (500 mMol) and TWEEN 20 (0.5 to 1.0%) 6. Bands larger than the target protein may be due to phosphorylation, glycosylation, splicing variants and additional cross-reactivity with known isoforms. Bands smaller than the target protein may be due to a cleaved fragment, protein degradation or the protein sample has been digested. 7. Many antibodies that have not been epitope affinity purified may produce non-specific bands and is to be expected. Unfortunately I do not have banding pattern data for endogenously expressed Cry1 at this time. 8. The protein may be forming multimers. Try boiling samples for 5-10 minutes to disrupt the multimers. I may be able to add the blocking peptide to our catalogue to determine between specific and non-specific bands, please let me know if you would be interested. I hope that these suggestions are helpful, please feel free to contact me with any additional questions that you may have or you still experience problems,

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Thank you for your enquiry. This antibody detects recombinant human cryptochrome 1 (Cry1). Ab3518 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~70 kDa protein representing full length recombinant human Cry1 protein.

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