Overview

  • Product name
    Anti-Cryptosporidium antibody [BEL 0126]
  • Description
    Mouse monoclonal [BEL 0126] to Cryptosporidium
  • Host species
    Mouse
  • Specificity
    This antibody recognizes a membrane antigen expressed by the oocysts of Cryptosporidium parvum
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Cryptosporidium
  • Immunogen

    Native, purified oocysts from bovine faeces

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    BEL 0126
  • Isotype
    IgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/8000.
ICC/IF 1/50 - 1/200.

Target

  • Relevance
    Cryptosporidium is a small protozoan parasite, measuring about 3-5 µm. It lives on (or just under) the surface of the cells lining the small intestine, reproduces asexually, and oocysts are passed in the faeces. Transmission of the infection occurs via the oocysts. Many human infections have been traced to the contamination of drinking water with oocysts from agricultural "run-off" (i.e., drainage from pastures), so it is considered a zoonosis. The most common symptom of cryptosporidiosis is watery diarrhoea; other symptoms include stomach cramps or pain, fever, nausea and vomiting. In persons with healthy immune systems, symptoms usually last about 1 to 2 weeks. Cryptosporidiosis is now recognized as an important opportunisitic infection, especially in immunocompromised hosts.

References

ab54066 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Thank you for your reply.

The volume added to the wells depends on the number of cells and surface available. It should cover all the cells surface completely.

You may want to try less concentrated antibody dilutions, for that, I would suggest trying first with a small amount of sample and antibody to verify the specific dilution is enough to provide a visible signal.

I hope this helps, otherwise, please do not hesitate to contact me back.

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Answer

Thank you for contacting us.

I appreciate the time taken to submit protocol information. The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would also appreciate if you could please send us an image and the order details.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab54066:

Fixing in paraformaldehyde for more than 10-15 min will cross link the proteins to the point where antigen retrieval may be required to ensure the antibody has free access to bind and detect the protein.



Instead, I would suggest fixation using 4% paraformaldehye to slides for 10 min only, or even better, trying acetone fixation, at -20C for 5-10 minutes. As this agent will also permeabilise, further permeabilization steps will not be required.

Use higher antibody dilutions (1/50) and use a positive control to assess the antibody’s performance.



I would suggest having a look at the following protocol, which will hopefully serve as a guidance to optimise the assay: https://www.abcam.com/index.html?pageconfig=resource&rid=11417

I hope this information is helpful. Should the suggestions not improve the results, please do not hesitate to contact me again and I will try to provide further help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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