Crystal violet Assay Kit (Cell viability) (ab232855)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Crystal violet Assay Kit (Cell viability) -
Detection method
Colorimetric -
Sample type
Adherent cells -
Species reactivity
Reacts with: Other species, Mammals -
Product overview
Crystal Violet Assay Kit ab232855 is used for cytoxicity and cell viability studies with adherent cell cultures.
The assay relies on the detachment of adherent cells from cell culture plates during cell death. During the assay, dead detached cells are washed away. The remaining attached cells are stained with Crystal violet, and after a wash step, the Crystal violet dye is solubilized and measured by absorbance at 595 nm.
The amount of Crystal violet staining in the assay is directly proportional to the cell biomass. The cell biomass can be used to infer levels of cell viability / cytotoxicity.
Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.
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Notes
Crystal violet is a triarylmethane dye that can bind to ribose type molecules such as DNA in nuclei.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1000 tests Crystal violet Staining Solution 1 x 40ml 10X Washing Solution 1 x 115ml Solubilization Solution 1 x 100ml 20 mM Doxorubicin 1 x 100µl
Images
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Dose-response curve of MCF7 (human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).Dose-response curve of MCF7 (Human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.
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Dose-response curve of HepG2 (human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).Dose-response curve of HepG2 (Human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.
Protocols
References
This product has been referenced in:
- Chowdhury KD et al. Cathepsin B mediated scramblase activation triggers cytotoxicity and cell cycle arrest by andrographolide to overcome cellular resistance in cisplatin resistant human hepatocellular carcinoma HepG2 cells. Environ Toxicol Pharmacol 68:120-132 (2019). Read more (PubMed: 30889542) »
