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    crystal-violet-assay-kit-cell-viability-ab232855.pdf

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Crystal violet Assay Kit (Cell viability) (ab232855)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (5)

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Dose-response curve of MCF7 (human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).
  • Dose-response curve of HepG2 (human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Adherent cells

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Overview

  • Product name

    Crystal violet Assay Kit (Cell viability)
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Crystal Violet Assay Kit ab232855 is used for cytoxicity and cell viability studies with adherent cell cultures.


    The Crystal Violet assay is based on staining cells that are attached to cell culture plates. It relies on the detachment of adherent cells from cell culture plates during cell death. During the assay, dead detached cells are washed away. The remaining attached live cells are stained with Crystal violet. After a wash step, the Crystal violet dye is solubilized and measured by absorbance at 570 nm.


    The amount of Crystal Violet staining in the assay is directly proportional to the cell biomass that is attached to the plate. The cell biomass can be used to infer levels of cell viability / cytotoxicity. The Crystal Violet assay assumes that all cells that are attached to the plate are "alive" and that all cells that detach are "dead".


    Crystal violet is a triarylmethane dye that binds to ribose type molecules such as DNA in nuclei.


    Crystal Violet assay protocol summary:
    - remove culture medium
    - wash cells
    - add Crystal Violet staining solution
    - incubate for 20 mins
    - wash cells and air dry plate
    - add solubilization solution
    - analyze using a microplate reader at 570 nm absorbance


     

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K329 Crystal Violet Cell Cytotoxicity Assay Kit. K329-1000 is the same size as the 1000 test size of ab232855.

    Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1000 tests
    Crystal violet Staining Solution 1 x 40ml
    10X Washing Solution 1 x 115ml
    Solubilization Solution 1 x 100ml
    20 mM Doxorubicin 1 x 100µl

Images

  • Dose-response curve of MCF7 (human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).
    Dose-response curve of MCF7 (human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).

    Dose-response curve of MCF7 (Human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.

  • Dose-response curve of HepG2 (human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).
    Dose-response curve of HepG2 (human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).

    Dose-response curve of HepG2 (Human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab232855? Please let us know so that we can cite the reference in this datasheet.

ab232855 has been referenced in 5 publications.

  • Puvvula PK & Moon AM Novel Cell-Penetrating Peptides Derived From Scaffold-Attachment- Factor A Inhibits Cancer Cell Proliferation and Survival. Front Oncol 11:621825 (2021). PubMed: 33859938
  • Mallis P  et al. Efficient differentiation of vascular smooth muscle cells from Wharton's Jelly mesenchymal stromal cells using human platelet lysate: A potential cell source for small blood vessel engineering. World J Stem Cells 12:203-221 (2020). PubMed: 32266052
  • Elmansi AM  et al. Age-related increase of kynurenine enhances miR29b-1-5p to decrease both CXCL12 signaling and the epigenetic enzyme Hdac3 in bone marrow stromal cells. Bone Rep 12:100270 (2020). PubMed: 32395570
  • Kuittinen T  et al. Paclitaxel, Carboplatin and 1,25-D3 Inhibit Proliferation of Ovarian Cancer Cells In Vitro. Anticancer Res 40:3129-3138 (2020). PubMed: 32487607
  • Chowdhury KD  et al. Cathepsin B mediated scramblase activation triggers cytotoxicity and cell cycle arrest by andrographolide to overcome cellular resistance in cisplatin resistant human hepatocellular carcinoma HepG2 cells. Environ Toxicol Pharmacol 68:120-132 (2019). PubMed: 30889542

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