Overview

  • Product name
    Anti-CRYZ antibody [EPR10843(B)]
    See all CRYZ primary antibodies
  • Description
    Rabbit monoclonal [EPR10843(B)] to CRYZ
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CRYZ (Uniprot: Q08257)

  • Positive control
    • Human fetal liver lysate; HepG2 and K562 cell lysates; Human pancreas tissue; Human thyroid gland carcinoma tissue; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
    • Tissue specificity
      Only very low amounts in the lens.
    • Sequence similarities
      Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Crystallin zeta (quinone reductase) antibody
      • Crystallin zeta antibody
      • Cryz antibody
      • DKFZp779E0834 antibody
      • FLJ41475 antibody
      • NADPH:quinone reductase antibody
      • QOR_HUMAN antibody
      • Quinone oxidoreductase antibody
      • Zeta crystallin antibody
      • Zeta-crystallin antibody
      see all

    Images

    • All lanes : Anti-CRYZ antibody [EPR10843(B)] (ab154842) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling CRYZ using ab154842 at a 1/100 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling CRYZ using ab154842 at a 1/100 dilution.
    • Immunofluorescence analysis of HepG2 cells, labeling CRYZ using ab154842 at a 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154842 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154842 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154842 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154842 showing +ve staining.

    References

    ab154842 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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