Anti-CRYZL1 antibody (ab224414)
Key features and details
- Rabbit polyclonal to CRYZL1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CRYZL1 antibody -
Description
Rabbit polyclonal to CRYZL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CRYZL1 aa 1-100.
Database link: O95825 -
Positive control
- WB: HEK-293T cells overexpressing recombinant CRYZL1 with C-terminal myc-DDK tag, cell lysate. IHC-P: Human testis and cerebral cortex tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 39 kDa.
|
Notes |
---|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 39 kDa. |
Target
-
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. - Information by UniProt
-
Database links
- Entrez Gene: 9946 Human
- Omim: 603920 Human
- SwissProt: O95825 Human
- Unigene: 352671 Human
-
Alternative names
- 4P11 antibody
- Crystallin zeta (quinone reductase) like 1 antibody
- CRYZL1 antibody
see all
Images
-
Paraffin-embedded human cerebral cortex tissue stained for CRYZL1 with ab224414 (1/20) in immunohistochemical analysis.
-
All lanes : Anti-CRYZL1 antibody (ab224414) at 1/100 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with vector only, cell lysate
Lane 2 : HEK-293T cells transfected with recombinant CRYZL1 with C-terminal myc-DDK tag (~3.1 kDa), cell lysate
Predicted band size: 39 kDa -
Paraffin-embedded human gastrointestinal tissue stained for CRYZL1 with ab224414 (1/20) in immunohistochemical analysis.
-
Paraffin-embedded human testis tissue stained for CRYZL1 with ab224414 (1/20) in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab224414 has not yet been referenced specifically in any publications.