Key features and details
- Rabbit polyclonal to CRYZL1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CRYZL1 antibody
DescriptionRabbit polyclonal to CRYZL1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: HEK-293T cells overexpressing recombinant CRYZL1 with C-terminal myc-DDK tag, cell lysate. IHC-P: Human testis and cerebral cortex tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 39 kDa.|
Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
- Information by UniProt
- 4P11 antibody
- Crystallin zeta (quinone reductase) like 1 antibody
- CRYZL1 antibody
Paraffin-embedded human cerebral cortex tissue stained for CRYZL1 with ab224414 (1/20) in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for CRYZL1 with ab224414 (1/20) in immunohistochemical analysis.
All lanes : Anti-CRYZL1 antibody (ab224414) at 1/100 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with vector only, cell lysate
Lane 2 : HEK-293T cells transfected with recombinant CRYZL1 with C-terminal myc-DDK tag (~3.1 kDa), cell lysate
Predicted band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224414 has not yet been referenced specifically in any publications.