Overview

  • Product name

  • Description

    Rabbit polyclonal to CSAD/CSD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse CSAD/CSD aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab103175)

  • Positive control

    • This antibody gave a positive signal in the following lysates: Liver (Mouse) Tissue; Kidney (Mouse) Tissue; Liver (Rat) Tissue; Kidney (Rat) Tissue; NIH 3T3 Whole Cell. IHC-P (FFPE): Rat Kidney (Normal)
  • General notes

     This product was previously labelled as CSAD

     

Properties

Applications

Our Abpromise guarantee covers the use of ab91016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-CSAD/CSD antibody (ab91016) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Mouse kidney tissue lysate

    Lysates/proteins at 50 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa


    Exposure time: 30 seconds

    See Abreview

  • IHC image of CSAD/CSD staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91016, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-CSAD/CSD antibody (ab91016) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Kidney (Rat) Tissue Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 100 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes
  • ICC/IF image of ab91016 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91016 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (5min) PC12 cells at 5ug/ml.

References

This product has been referenced in:

  • Aumailley L  et al. Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional Werner syndrome (Wrn) mutant protein. PLoS One 13:e0193170 (2018). Read more (PubMed: 29494634) »
  • Wang Y  et al. Bile acids regulate cysteine catabolism and glutathione regeneration to modulate hepatic sensitivity to oxidative injury. JCI Insight 3:N/A (2018). Read more (PubMed: 29669937) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12 % gel)
Sample
Mouse Tissue lysate - other (liver and kidney)
Specification
liver and kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Hua Jiang

Verified customer

Submitted Jun 17 2013

Question
Answer

Thank you for your enquiry.

The lab used 5% BSA for testing purposes and for the picture display on this webpage.



Please let us know if you have further questions.

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