Anti-CSAD/CSD antibody (ab91016)
Key features and details
- Rabbit polyclonal to CSAD/CSD
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-CSAD/CSD antibody -
Description
Rabbit polyclonal to CSAD/CSD -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse CSAD/CSD aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103175) -
Positive control
- WB: Mouse and rat liver and kidney tissue lysates. ICC/IF: PC-12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Pathway
Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 2/2. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Database links
- Entrez Gene: 246277 Mouse
- Entrez Gene: 60356 Rat
- SwissProt: Q9DBE0 Mouse
- SwissProt: Q64611 Rat
- Unigene: 296382 Mouse
- Unigene: 43232 Rat
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Alternative names
- Csad antibody
- CSAD_HUMAN antibody
- CSD antibody
see all
Images
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All lanes : Anti-CSAD/CSD antibody (ab91016) at 1 µg/ml
Lane 1 : Mouse Liver lysate
Lane 2 : Mouse Kidney lysate
Lane 3 : Rat Liver lysate
Lane 4 : Rat Kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA before being incubated with ab91016 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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ICC/IF image of ab91016 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91016 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (5min) PC12 cells at 5ug/ml.
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All lanes : Anti-CSAD/CSD antibody (ab91016) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lysates/proteins at 50 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab91016 has been referenced in 7 publications.
- Zhang X et al. Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma. Cancer Commun (Lond) 42:287-313 (2022). PubMed: 35184419
- Guerra DD et al. Estrogen Regulates Local Cysteine Metabolism in Mouse Myometrium. Reprod Sci 28:79-90 (2021). PubMed: 32820455
- Aumailley L et al. Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional Werner syndrome (Wrn) mutant protein. PLoS One 13:e0193170 (2018). PubMed: 29494634
- Wang Y et al. Bile acids regulate cysteine catabolism and glutathione regeneration to modulate hepatic sensitivity to oxidative injury. JCI Insight 3:N/A (2018). PubMed: 29669937
- Wang Y et al. HNF4a Regulates CSAD to Couple Hepatic Taurine Production to Bile Acid Synthesis in Mice. Gene Expr 18:187-196 (2018). PubMed: 29871716
- Jiang H et al. Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap. Biol Open 4:1154-62 (2015). PubMed: 26276101
- Jiang H et al. Altered hepatic sulfur metabolism in cystathionine ß-synthase-deficient homocystinuria: regulatory role of taurine on competing cysteine oxidation pathways. FASEB J 28:4044-54 (2014). PubMed: 24891521