Recombinant Anti-CSC1 antibody [EPR19814] (ab203486)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19814] to CSC1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CSC1 antibody [EPR19814]
See all CSC1 primary antibodies -
Description
Rabbit monoclonal [EPR19814] to CSC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment within Mouse CSC1 aa 200-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8CBX0
Recombinant fragment within Mouse CSC1 aa 700 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8CBX0 -
Positive control
- WB: Mouse and rat cerebral cortex and cerebellum lysates; CSC1 WT (+/+) mouse brain lysate. ICC/IF: mCSC1 transfected HEK-293T cells. IP: Mouse and rat cerebellum lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19814 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
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ICC/IF |
1/150.
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IP |
1/40.
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Notes |
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WB
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa). |
ICC/IF
1/150. |
IP
1/40. |
Target
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Function
Acts as an osmosensitive calcium-permeable cation channel. -
Sequence similarities
Belongs to the CSC1 (TC 1.A.17) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57156 Human
- Entrez Gene: 217733 Mouse
- Entrez Gene: 314332 Rat
- SwissProt: Q9P1W3 Human
- SwissProt: Q8CBX0 Mouse
- Unigene: 22452 Human
- Unigene: 110032 Mouse
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Alternative names
- 4932420N09 antibody
- 9330187M14Rik antibody
- C14orf171 antibody
see all
Images
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All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/1000 dilution
Lane 1 : Mouse cerebral cortex tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Rat cerebellum tissue lysate
Lane 4 : Rat cerebral cortex tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2, 3 and 4: 10 seconds.
To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.
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All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/400 dilution
Lane 1 : CSC1 KO (-/-) mouse brain lysate
Lane 2 : CSC1 WT (+/+) mouse brain lysate
Lane 3 : CSC1 heterozygous (+/-) mouse brain lysate
Secondary
All lanes : Goat Anti-Mouse IgG (H+L) HRP at 1/15000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red).
Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
The nuclear counter stain is DAPI (blue).
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CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse cerebellum lysate 10 μg (Input).
Lane 2: ab203486 IP in mouse cerebellum lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in mouse cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.
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CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Rat cerebellum lysate 10 μg (Input).
Lane 2: ab203486 IP in rat cerebellum lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in rat cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab203486 has been referenced in 1 publication.
- Tábara LC et al. TMEM63C mutations cause mitochondrial morphology defects and underlie hereditary spastic paraplegia. Brain 145:3095-3107 (2022). PubMed: 35718349