Recombinant
RabMAb

Recombinant Anti-CSC1 antibody [EPR19814] (ab203486)

Overview

  • Product name

    Anti-CSC1 antibody [EPR19814]
    See all CSC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19814] to CSC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment within Mouse CSC1 aa 1-100. The exact sequence is proprietary.
    Database link: Q8CBX0

    Recombinant fragment within Mouse CSC1 aa 200-400. The exact sequence is proprietary.
    Database link: Q8CBX0

    Recombinant fragment within Mouse CSC1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8CBX0

  • Positive control

    • WB: Mouse and rat cerebral cortex and cerebellum lysates; CSC1 WT (+/+) mouse brain lysate. ICC/IF: mCSC1 transfected HEK-293T cells. IP: Mouse and rat cerebellum lysates.
  • General notes

     This product was previously labelled as TMEM63C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19814
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
ICC/IF 1/150.
IP 1/40.

Target

  • Function

    Acts as an osmosensitive calcium-permeable cation channel.
  • Sequence similarities

    Belongs to the CSC1 (TC 1.A.17) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4932420N09 antibody
    • 9330187M14Rik antibody
    • C14orf171 antibody
    • Calcium permeable stress gated cation channel 1 homolog antibody
    • Calcium permeable stress-gated cation channel 1 antibody
    • Chromosome 14 open reading frame 171 antibody
    • CSC1 antibody
    • CSC1_HUMAN antibody
    • DKFZp434P0111 antibody
    • hsCSC1 antibody
    • RGD1310207 antibody
    • TMEM63C antibody
    • Transmembrane protein 63C antibody
    see all

Images

  • All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/1000 dilution

    Lane 1 : Mouse cerebral cortex lysate
    Lane 2 : Mouse cerebellum lysate
    Lane 3 : Rat cerebellum lysate
    Lane 4 : Rat cerebral cortex lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2, 3 and 4: 10 seconds.

    To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.

  • All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/400 dilution

    Lane 1 : CSC1 KO (-/-) mouse brain lysates
    Lane 2 : CSC1 WT (+/+) mouse brain lysates
    Lane 3 : CSC1 heterozygous (+/-) mouse brain lysates

    Secondary
    All lanes : Goat Anti-Mouse IgG(H+L) HRP at 1/15000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The Images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University, MARC.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red).

    Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

    The nuclear counter stain is DAPI (blue).

     

  • CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse cerebellum lysate 10 μg (Input).

    Lane 2: ab203486 IP in mouse cerebellum lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in mouse cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

  • CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Rat cerebellum lysate 10 μg (Input).

    Lane 2: ab203486 IP in rat cerebellum lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in rat cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

References

ab203486 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (6% gel)
Loading amount
40 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Dr. guifang duan

Verified customer

Submitted Sep 20 2017

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