Recombinant Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
![Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)](https://www.abcam.com/ps/products/251/ab251395/Images/ab251395-410566-anti-csc1-antibody-epr19814-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19814] to CSC1 - BSA and Azide free
- Suitable for: WB, IP, ICC
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CSC1 antibody [EPR19814] - BSA and Azide free
See all CSC1 primary antibodies -
Description
Rabbit monoclonal [EPR19814] to CSC1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP RatWB Mouse
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Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment within Mouse CSC1 aa 200-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8CBX0
Recombinant fragment within Mouse CSC1 aa 700 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8CBX0 -
General notes
ab251395 is the carrier-free version of ab203486 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab251395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as TMEM63C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19814 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Rat
|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa). |
|
IP
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
Target
-
Function
Acts as an osmosensitive calcium-permeable cation channel. -
Sequence similarities
Belongs to the CSC1 (TC 1.A.17) family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 217733 Mouse
- Entrez Gene: 314332 Rat
- SwissProt: Q8CBX0 Mouse
- Unigene: 110032 Mouse
-
Alternative names
- 4932420N09 antibody
- 9330187M14Rik antibody
- C14orf171 antibody
see all
Images
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Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/1000 dilution
Lane 1 : Mouse cerebral cortex tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Rat cerebellum tissue lysate
Lane 4 : Rat cerebral cortex tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDaThis data was developed using ab203486, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 minutes; Lanes 2-4: 10 seconds.
To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.
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![Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)](https://www.abcam.com/ps/products/251/ab251395/Images/ab251395-408234-anti-csc1-antibody-epr19814-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)The Images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University, MARC.All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/400 dilution
Lane 1 : CSC1 KO (-/-) mouse brain lysate
Lane 2 : CSC1 WT (+/+) mouse brain lysate
Lane 3 : CSC1 heterozygous (+/-) mouse brain lysate
Secondary
All lanes : Goat Anti-Mouse IgG (H+L) HRP at 1/15000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 30 secondsThis data was developed using ab203486, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)](https://www.abcam.com/ps/products/251/ab251395/Images/ab251395-3-anti-csc1-antibody-epr19814-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)This data was developed using ab203486, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red). Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC. The nuclear counter stain is DAPI (blue). -
![Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)](https://www.abcam.com/ps/products/251/ab251395/Images/ab251395-2-anti-csc1-antibody-epr19814-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)This data was developed using ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse cerebellum lysate 10 μg (Input). Lane 2: ab203486 IP in mouse cerebellum lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in mouse cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates. -
![Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)](https://www.abcam.com/ps/products/251/ab251395/Images/ab251395-1-anti-csc1-antibody-epr19814-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)This data was developed using ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Rat cerebellum lysate 10 μg (Input). Lane 2: ab203486 IP in rat cerebellum lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in rat cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates. -
![Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)](https://www.abcam.com/ps/products/251/ab251395/Images/ab251395-6-benefits-of-recombinant-antibodies.png)
Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab251395 has not yet been referenced specifically in any publications.
