For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    csc1-antibody-epr19814-bsa-and-azide-free-ab251395.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Other Antibodies Other Antibodies
Share by email
RecombinantRabMAb

Recombinant Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
  • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
  • Immunocytochemistry - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
  • Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19814] to CSC1 - BSA and Azide free
  • Suitable for: WB, IP, ICC
  • Reacts with: Mouse, Rat

You may also be interested in

Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-CSC1 antibody [EPR19814] - BSA and Azide free
    See all CSC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19814] to CSC1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Rat
    WB
    Mouse
    See all applications and species data
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment within Mouse CSC1 aa 200-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8CBX0

    Recombinant fragment within Mouse CSC1 aa 700 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8CBX0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    ab251395 is the carrier-free version of ab203486 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab251395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TMEM63C

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR19814
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Alternative Versions

    • Anti-CSC1 antibody [EPR19814] (ab203486)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Rat
WB
Mouse
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

Target

  • Function

    Acts as an osmosensitive calcium-permeable cation channel.
  • Sequence similarities

    Belongs to the CSC1 (TC 1.A.17) family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9P1W3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 217733 Mouse
    • Entrez Gene: 314332 Rat
    • SwissProt: Q8CBX0 Mouse
    • Unigene: 110032 Mouse
    • Alternative names

      • 4932420N09 antibody
      • 9330187M14Rik antibody
      • C14orf171 antibody
      • Calcium permeable stress gated cation channel 1 homolog antibody
      • Calcium permeable stress-gated cation channel 1 antibody
      • Chromosome 14 open reading frame 171 antibody
      • CSC1 antibody
      • CSC1_HUMAN antibody
      • DKFZp434P0111 antibody
      • hsCSC1 antibody
      • RGD1310207 antibody
      • TMEM63C antibody
      • Transmembrane protein 63C antibody
      see all

    Images

    • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/1000 dilution

      Lane 1 : Mouse cerebral cortex tissue lysate
      Lane 2 : Mouse cerebellum tissue lysate
      Lane 3 : Rat cerebellum tissue lysate
      Lane 4 : Rat cerebral cortex tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 93 kDa
      Observed band size: 93 kDa



      This data was developed using ab203486, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 3 minutes; Lanes 2-4: 10 seconds.

      To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.

    • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)The Images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University, MARC.
      All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/400 dilution

      Lane 1 : CSC1 KO (-/-) mouse brain lysate
      Lane 2 : CSC1 WT (+/+) mouse brain lysate
      Lane 3 : CSC1 heterozygous (+/-) mouse brain lysate

      Secondary
      All lanes : Goat Anti-Mouse IgG (H+L) HRP at 1/15000 dilution

      Predicted band size: 93 kDa
      Observed band size: 93 kDa


      Exposure time: 30 seconds


      This data was developed using ab203486, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      Immunocytochemistry - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      This data was developed using ab203486, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red). Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC. The nuclear counter stain is DAPI (blue).
    • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      This data was developed using ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse cerebellum lysate 10 μg (Input). Lane 2: ab203486 IP in mouse cerebellum lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in mouse cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.
    • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      This data was developed using ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Rat cerebellum lysate 10 μg (Input). Lane 2: ab203486 IP in rat cerebellum lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in rat cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.
    • Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
      Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab251395? Please let us know so that we can cite the reference in this datasheet.

    ab251395 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab251395.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.