Overview

  • Product name

  • Description

    Rabbit polyclonal to CSDE1/NRU
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human CSDE1/NRU aa 167-372. (AAH32446)

  • Positive control

    • HeLa whole cell lysate (ab150035); A549, HepG2 and HCT116 lysates
  • General notes

     This product was previously labelled as CSDE1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
  • Sequence similarities

    Contains 9 CSD (cold-shock) domains.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cold shock domain containing E1 RNA binding antibody
    • Cold shock domain containing protein E1 antibody
    • Cold shock domain-containing protein E1 antibody
    • Csde1 antibody
    • CSDE1_HUMAN antibody
    • D1S155E antibody
    • DKFZp779B0247 antibody
    • DKFZp779J1455 antibody
    • FLJ26882 antibody
    • N-ras upstream gene protein antibody
    • NRAS related antibody
    • Nras upstream gene protein antibody
    • NRU antibody
    • Protein UNR antibody
    • RP5 1000E10.3 antibody
    • UNR antibody
    • UNR protein antibody
    • Upstream of NRAS antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab96124 at 1:250 dilution. 
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • All lanes : Anti-CSDE1/NRU antibody (ab96124) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : The HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 88 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa, using ab96124 antibody at 1:200 dilution. Costained with Hoechst 33342.

  • Anti-CSDE1/NRU antibody (ab96124) at 1/3000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 88 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Martinez-Useros J  et al. UNR/CDSE1 expression as prognosis biomarker in resectable pancreatic ductal adenocarcinoma patients: A proof-of-concept. PLoS One 12:e0182044 (2017). Read more (PubMed: 28763470) »
  • Ju Lee H  et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun 8:1456 (2017). Read more (PubMed: 29129916) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (melanoma)
Antigen retrieval step
Other - Buffer/Enzyme Used: high pH buffered solution (dako system)
Permeabilization
No
Specification
melanoma
Fixative
Formalin

Abcam user community

Verified customer

Submitted Feb 07 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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