Recombinant Anti-CSDE1/NRU antibody [EPR17413] (ab200663)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17413] to CSDE1/NRU
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CSDE1/NRU antibody [EPR17413]
See all CSDE1/NRU primary antibodies -
Description
Rabbit monoclonal [EPR17413] to CSDE1/NRU -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, HeLa, MCF7, C6, RAW 264.7 and PC-12 cell lysates; Human fetal brain lysate; HeLa cytosolic fraction. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17413 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
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IP |
1/140.
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Notes |
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WB
1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). |
IP
1/140. |
Target
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Function
RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. -
Sequence similarities
Contains 9 CSD (cold-shock) domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7812 Human
- Entrez Gene: 229663 Mouse
- Entrez Gene: 117180 Rat
- Omim: 191510 Human
- SwissProt: O75534 Human
- SwissProt: Q91W50 Mouse
- SwissProt: P18395 Rat
- Unigene: 69855 Human
see all -
Alternative names
- Cold shock domain containing E1 RNA binding antibody
- Cold shock domain containing protein E1 antibody
- Cold shock domain-containing protein E1 antibody
see all
Images
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All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution + MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution + Human fetal brain tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution
Lane 1 : HeLa cytosolic fraction
Lane 2 : HeLa membrane (mitochondrial) fraction
Lane 3 : HeLa nuclear fraction
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The HeLa cell fractions lysate set is ab168542 from Abcam.
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CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab200663 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/1000 dilution (Panel A) or ab201688 at 1/1000 dilution (Panel B). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab200663 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 min (Panel A); 10 seconds (Panel B).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab200663 has been referenced in 1 publication.
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708