Overview

  • Product name

    Anti-CSDE1/NRU antibody [EPR17414]
    See all CSDE1/NRU primary antibodies
  • Description

    Rabbit monoclonal [EPR17414] to CSDE1/NRU
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CSDE1/NRU aa 400-500. The exact sequence is proprietary.
    Database link: O75534

  • Positive control

    • WB: K562, HEK293, Raw264.7 and HeLa cell lysate. Human testis, mouse and rat lysate. IHC-P: Human breast carcinoma and Human kidney tissue. ICC/IF: HeLa and MCF7 cells. IP: K562 cells.
  • General notes

     

     

     This product was previously labelled as CSDE1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IP 1/40.
WB 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
  • Sequence similarities

    Contains 9 CSD (cold-shock) domains.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cold shock domain containing E1 RNA binding antibody
    • Cold shock domain containing protein E1 antibody
    • Cold shock domain-containing protein E1 antibody
    • Csde1 antibody
    • CSDE1_HUMAN antibody
    • D1S155E antibody
    • DKFZp779B0247 antibody
    • DKFZp779J1455 antibody
    • FLJ26882 antibody
    • N-ras upstream gene protein antibody
    • NRAS related antibody
    • Nras upstream gene protein antibody
    • NRU antibody
    • Protein UNR antibody
    • RP5 1000E10.3 antibody
    • UNR antibody
    • UNR protein antibody
    • Upstream of NRAS antibody
    see all

Images

  • All lanes : Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/10000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) lysate
    Lane 2 : HEK293 (Human embryonic kidney)
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/1000 dilution + Human testis at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/1000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Rat brain
    Lane 3 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasmic staining on Human breast carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human kidney tissue is observed.

    Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). 

    Confocal image showing cytoplasmic staining on HeLa cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing cytoplasmic staining on MCF7 cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • CSDE1/NRU was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab201688 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab201688 at 1/1500 dilution. 

    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).

    Lane 2: ab201688 IP in K562 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20688 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

References

This product has been referenced in:

  • Booy EP  et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). Read more (PubMed: 30247708) »
  • Ju Lee H  et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun 8:1456 (2017). Read more (PubMed: 29129916) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201688.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up