• Product name
  • Description
    Rabbit polyclonal to CSEN
  • Host species
  • Specificity
    ab61770 detects endogenous levels of total CSEN protein
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human CSEN around the phosphorylation site of serine 63 (D-S-SP-D-S).

  • Positive control
    • Human brain tissue; extracts from A431 cells



Our Abpromise guarantee covers the use of ab61770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.


  • Function
    Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
    May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.
  • Tissue specificity
    Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
  • Sequence similarities
    Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Post-translational
    Palmitoylated. Palmitoylation enhances association with the plasma membrane.
    Proteolytically cleaved by caspase-3.
    Phosphorylation at Ser-63 inhibits cleavage by CASP3.
  • Cellular localization
    Cytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933407H12Rik antibody
    • A type potassium channel modulatory protein 3 antibody
    • A-type potassium channel modulatory protein 3 antibody
    • AI413860 antibody
    • Calsenilin antibody
    • CSEN antibody
    • CSEN_HUMAN antibody
    • DRE antagonist modulator antibody
    • DRE-antagonist modulator antibody
    • DREAM antibody
    • EF hand transcription factor antibody
    • KCHIP 3 antibody
    • KChIP3 antibody
    • KCNIP 3 antibody
    • Kcnip3 antibody
    • Kv channel interacting protein 3 antibody
    • Kv channel-interacting protein 3 antibody
    • MGC18289 antibody
    • Potassium channel interacting protein 3 antibody
    • R74849 antibody
    see all


  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61770 at a dilution of 1/50. left: untreated sample. Right: sample treated with immunising peptide.
  • All lanes : Anti-CSEN antibody (ab61770) at 1/500 dilution

    Lane 1 : extracts from A431 cells with no peptide
    Lane 2 : extracts from A431 cells with peptide

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • ICC/IF image of ab61770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61770, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab61770 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Western blot
Escherichia coli Purified protein (E.Coli)
Loading amount
0.05 µg
Gel Running Conditions
Reduced Denaturing (gradient 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Submitted Jan 09 2009


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