Product nameAnti-CSF-1-R antibody
See all CSF-1-R primary antibodies
DescriptionRabbit polyclonal to CSF-1-R
ab59231 detects endogenous levels of total CSF-1-R protein.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human CSF-1-R. Synthetic non-phosphopeptide derived from human CSF-1-R around the phosphorylation site of tyrosine 809 (S-N-YP-V-V).
- WB: 293 cell extracts treated with LPS (100ng/ml, 30mins).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab59231 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab59231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 46 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProtein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
Tissue specificityExpressed in bone marrow and in differentiated blood mononuclear cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
- C FMS antibody
- CD 115 antibody
- CD115 antibody
All lanes : Anti-CSF-1-R antibody (ab59231) at 1/500 dilution
Lane 1 : 293 cell extracts treated
with LPS (100ng/ml, 30mins)
Lane 2 : 293 cell extracts treated
with LPS (100ng/ml, 30mins) with immunising peptide
Predicted band size: 46 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab59231 staining Human placenta. Staining is localised to the cell membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab59231 has not yet been referenced specifically in any publications.