RecombinantRabMAb

Recombinant Anti-CSF-1-R antibody [EPR20754] (ab229188)

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Western blot - Anti-CSF-1-R antibody [EPR20754] (ab229188)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)
  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] (ab229188)
  • Anti-CSF-1-R antibody [EPR20754] (ab229188)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20754] to CSF-1-R
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CSF-1-R antibody [EPR20754]
    See all CSF-1-R primary antibodies

  • Description

    Rabbit monoclonal [EPR20754] to CSF-1-R

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta and tonsil lysates. IHC-P: Human tonsil, non-Hodgkin lymphoma and lung cancer tissues. IP: Human tonsil lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab229188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/30.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Notes
IP
1/30.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

Target

  • Function

    Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.

  • Tissue specificity

    Expressed in bone marrow and in differentiated blood mononuclear cells.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • C FMS antibody
      • CD 115 antibody
      • CD115 antibody
      • CD115 antigen antibody
      • CFMS antibody
      • Colony stimulating factor 1 receptor antibody
      • Colony stimulating factor I receptor antibody
      • CSF 1 R antibody
      • CSF 1R antibody
      • CSF-1 receptor antibody
      • CSF-1-R antibody
      • CSF1 R antibody
      • CSF1R antibody
      • CSF1R_HUMAN antibody
      • CSFR antibody
      • EC 2.7.10.1 antibody
      • FIM 2 antibody
      • FIM2 antibody
      • FMS antibody
      • FMS proto oncogene antibody
      • FMS protooncogene antibody
      • HDLS antibody
      • M-CSF Receptor antibody
      • M-CSF-R antibody
      • Macrophage colony stimulating factor 1 receptor antibody
      • Macrophage colony stimulating factor I receptor antibody
      • Macrophage colony-stimulating factor 1 receptor antibody
      • McDonough feline sarcoma viral (v fms) oncogene homolog antibody
      • MCSFR antibody
      • Oncogen FMS antibody
      • Proto-oncogene c-Fms antibody
      • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly antibody
      see all

    Images

    • Western blot - Anti-CSF-1-R antibody [EPR20754] (ab229188)
      Western blot - Anti-CSF-1-R antibody [EPR20754] (ab229188)
      All lanes : Anti-CSF-1-R antibody [EPR20754] (ab229188) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human tonsil tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 108 kDa
      Observed band size: 108 kDa


      Exposure time: 3 minutes


      Blocking/dilution buffer: 5% NFDM/TBST.

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)

      Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages infiltrating human lung cancer is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)

      Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic and membranous staining of human non-Hodgkin lymphoma cells is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (ab229188)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages of human tonsil (PMID: 26066800) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] (ab229188)
      Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] (ab229188)

      CSF-1-R was immunoprecipitated from 0.35 mg of human tonsil lysate with ab229188 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229188 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Human tonsil lysate 10 µg (Input).
      Lane 2: ab229188 IP in human tonsil lysate. 
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229188 in human tonsil lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Anti-CSF-1-R antibody [EPR20754] (ab229188)
      Anti-CSF-1-R antibody [EPR20754] (ab229188)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab229188? Please let us know so that we can cite the reference in this datasheet.

    ab229188 has been referenced in 1 publication.

    • Liu B  et al. Variants Affecting the C-Terminal of CSF1R Cause Congenital Vertebral Malformation Through a Gain-of-Function Mechanism. Front Cell Dev Biol 9:641133 (2021). PubMed: 33816491

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