Recombinant Anti-CSF-1-R antibody [EPR21885-161] (ab221684)
![Western blot - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)](https://www.abcam.com/ps/products/221/ab221684/Images/ab221684-308550-anti-mcsf-receptor-antibody-epr21885-161-western-blot.jpg "Western blot - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21885-161] to CSF-1-R
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CSF-1-R antibody [EPR21885-161]
See all CSF-1-R primary antibodies -
Description
Rabbit monoclonal [EPR21885-161] to CSF-1-R -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Mouse CSF-1-R aa 1-550. The exact sequence is proprietary.
Database link: P09581 -
Positive control
- WB: Rat lung lysate, Mouse spleen and placenta lysate, J774A.1 and RAW 264.7 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21885-161 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab221684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 108 kDa. |
Target
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12978 Mouse
- Entrez Gene: 307403 Rat
- SwissProt: P09581 Mouse
- SwissProt: Q00495 Rat
- Unigene: 22574 Mouse
- Unigene: 72599 Rat
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Alternative names
- C FMS antibody
- CD 115 antibody
- CD115 antibody
see all
Images
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Western blot - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)All lanes : Anti-CSF-1-R antibody [EPR21885-161] (ab221684) at 1/1000 dilution
Lane 1 : Rat lung lysate at 20 µg
Lane 2 : Mouse spleen lysate at 20 µg
Lane 3 : Mouse placenta lysate at 20 µg
Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 10 µg
Lane 5 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Predicted band size: 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 3 minutes; Lane 5: 32 seconds.
The molecular mass observed is consistent with the literature (PMID 21610095; PMID 20436485; PMID 24362524; PMID: 26066800).
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![Anti-CSF-1-R antibody [EPR21885-161] (ab221684)](https://www.abcam.com/ps/products/221/ab221684/Images/ab221684-2-benefits-of-recombinant-antibodies.png)
Anti-CSF-1-R antibody [EPR21885-161] (ab221684)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab221684 has not yet been referenced specifically in any publications.
