Recombinant Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)
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Overview
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Product name
Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free
See all CSF-1-R primary antibodies -
Description
Rabbit monoclonal [EPR21885-161] to CSF-1-R - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Mouse CSF-1-R aa 1-550. The exact sequence is proprietary.
Database link: P09581 -
Positive control
- WB: Rat lung lysate, Mouse spleen and placenta lysate, J774A.1 and RAW 264.7 whole cell lysate.
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General notes
Ab234525 is the carrier-free version of ab221684. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab234525 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21885-161 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab234525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 108 kDa. |
Target
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12978 Mouse
- Entrez Gene: 307403 Rat
- SwissProt: P09581 Mouse
- SwissProt: Q00495 Rat
- Unigene: 22574 Mouse
- Unigene: 72599 Rat
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Alternative names
- C FMS antibody
- CD 115 antibody
- CD115 antibody
see all
Images
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![Western blot - Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)](https://www.abcam.com/ps/products/234/ab234525/Images/ab234525-308568-anti-mcsf-receptor-antibody-epr21885-161-western-blot.jpg)
Western blot - Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)All lanes : Anti-CSF-1-R antibody [EPR21885-161] (ab221684) at 1/1000 dilution
Lane 1 : Rat lung lysate at 20 µg
Lane 2 : Mouse spleen lysate at 20 µg
Lane 3 : Mouse placenta lysate at 20 µg
Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 10 µg
Lane 5 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Predicted band size: 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 3 minutes; Lane 5: 32 seconds.
The molecular mass observed is consistent with the literature (PMID 21610095; PMID 20436485; PMID 24362524; PMID: 26066800).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221684).
Datasheets and documents
References
ab234525 has not yet been referenced specifically in any publications.
