RecombinantRabMAb

Recombinant Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23529-26] to CSF-1-R - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free
    See all CSF-1-R primary antibodies

  • Description

    Rabbit monoclonal [EPR23529-26] to CSF-1-R - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7, J774A.1 and Mouse spleen, Mouse placenta lysates. IHC-P: and Mouse spleen, Mouse liver tissues. ICC/IF: RAW264.7 cell. IP: RAW 264.7 cell.

  • General notes

    ab272049 is the carrier-free version of ab254357.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Function

    Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.

  • Tissue specificity

    Expressed in bone marrow and in differentiated blood mononuclear cells.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • C FMS antibody
      • CD 115 antibody
      • CD115 antibody
      • CD115 antigen antibody
      • CFMS antibody
      • Colony stimulating factor 1 receptor antibody
      • Colony stimulating factor I receptor antibody
      • CSF 1 R antibody
      • CSF 1R antibody
      • CSF-1 receptor antibody
      • CSF-1-R antibody
      • CSF1 R antibody
      • CSF1R antibody
      • CSF1R_HUMAN antibody
      • CSFR antibody
      • EC 2.7.10.1 antibody
      • FIM 2 antibody
      • FIM2 antibody
      • FMS antibody
      • FMS proto oncogene antibody
      • FMS protooncogene antibody
      • HDLS antibody
      • M-CSF Receptor antibody
      • M-CSF-R antibody
      • Macrophage colony stimulating factor 1 receptor antibody
      • Macrophage colony stimulating factor I receptor antibody
      • Macrophage colony-stimulating factor 1 receptor antibody
      • McDonough feline sarcoma viral (v fms) oncogene homolog antibody
      • MCSFR antibody
      • Oncogen FMS antibody
      • Proto-oncogene c-Fms antibody
      • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in mouse spleen. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7 cells labelling CSF-1-R with ab254357 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      CSF-1-R was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug with ab254357 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug

      Lane 2: ab254357 IP in RAW 264.7 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254357 in RAW 264.7 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 128 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells in mouse liver. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab272049? Please let us know so that we can cite the reference in this datasheet.

    ab272049 has not yet been referenced specifically in any publications.

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