Recombinant Anti-CSF-1-R antibody [SP211] (ab183316)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-9-AntiMCSF-Receptor-antibody-SP211-IHC-rat-spleen.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP211] to CSF-1-R
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CSF-1-R antibody [SP211]
See all CSF-1-R primary antibodies -
Description
Rabbit monoclonal [SP211] to CSF-1-R -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon, lung, liver, skin, tonsil and skin squamous cell carcinoma, rat spleen tissues. Flow Cyt (intra): SK-BR-3 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP211 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
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| IHC-P | (3) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/20. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
Target
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1436 Human
- Entrez Gene: 12978 Mouse
- Entrez Gene: 307403 Rat
- Omim: 164770 Human
- SwissProt: P07333 Human
- SwissProt: P09581 Mouse
- SwissProt: Q00495 Rat
- Unigene: 586219 Human
see all -
Alternative names
- C FMS antibody
- CD 115 antibody
- CD115 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling CSF-1-R with ab183316 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183316 for 10 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-8-AntiMCSF-Receptor-antibody-SP211-IHC-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CSF-1-R with ab183316 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183316 for 10 mins at room temperature. -
![Flow Cytometry (Intracellular) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-330593-anti-mcsf-receptor-antibody-sp211-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CSF-1-R antibody [SP211] (ab183316)Intracellular Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma) labeling CSF-1-R with purified ab183316 at 1/20 dilution (5.65µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-213550-anti-mcsf-receptor-antibody-sp211-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)Immunohistochemical analysis of human tonsil tissue labeling CSF-1-R using ab183316 at a 1/100 dilution
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-213549-anti-mcsf-receptor-antibody-sp211-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)Immunohistochemical analysis of human skin tissue labeling CSF-1-R using ab183316 at a 1/100 dilution
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-213548-anti-mcsf-receptor-antibody-sp211-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)Immunohistochemical analysis of human skin squamous cell carcinoma tissue labeling CSF-1-R using ab183316 at a 1/100 dilution
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-213547-anti-mcsf-receptor-antibody-sp211-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)Immunohistochemical analysis of human colon tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-213546-anti-mcsf-receptor-antibody-sp211-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)Immunohistochemical analysis of human lung tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-213545-anti-mcsf-receptor-antibody-sp211-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)Immunohistochemical analysis of human liver tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.
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![Anti-CSF-1-R antibody [SP211] (ab183316)](https://www.abcam.com/ps/products/183/ab183316/Images/ab183316-10-benefits-of-recombinant-antibodies.png)
Anti-CSF-1-R antibody [SP211] (ab183316)
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab183316 has been referenced in 10 publications.
- Tang F et al. Patient-Derived Tumor Xenografts Plus Ex Vivo Models Enable Drug Validation for Tenosynovial Giant Cell Tumors. Ann Surg Oncol N/A:N/A (2021). PubMed: 33748895
- Shi X et al. Characterization of a p53/miR-34a/CSF1R/STAT3 Feedback Loop in Colorectal Cancer. Cell Mol Gastroenterol Hepatol 10:391-418 (2020). PubMed: 32304779
- Cassetta L et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell 35:588-602.e10 (2019). PubMed: 30930117
- Kitadate Y et al. Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche. Cell Stem Cell 24:79-92.e6 (2019). PubMed: 30581080
- Rashid R et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). PubMed: 31848351
- Weber M et al. Differences in Inflammation and Bone Resorption between Apical Granulomas, Radicular Cysts, and Dentigerous Cysts. J Endod 45:1200-1208 (2019). PubMed: 31400944
- Gurevich DB et al. Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression. EMBO J 37:N/A (2018). PubMed: 29866703
- Komohara Y et al. Potential anti-lymphoma effect of M-CSFR inhibitor in adult T-cell leukemia/lymphoma. J Clin Exp Hematop 58:152-160 (2018). PubMed: 30541986
- Olmeda D et al. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine. Nature 546:676-680 (2017). PubMed: 28658220
- Guo X et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). IHC, ICC/IF ; Human . PubMed: 27602954