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    csf-1-r-antibody-sp211-ab183316.pdf

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RecombinantRabMAb

Recombinant Anti-CSF-1-R antibody [SP211] (ab183316)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Flow Cytometry (Intracellular) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
  • Anti-CSF-1-R antibody [SP211] (ab183316)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP211] to CSF-1-R
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-CSF-1-R antibody [SP211]
    See all CSF-1-R primary antibodies
  • Description

    Rabbit monoclonal [SP211] to CSF-1-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon, lung, liver, skin, tonsil and skin squamous cell carcinoma, rat spleen tissues. Flow Cyt (intra): SK-BR-3 cells.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP211
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Other
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • CSFs
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-CSF-1-R antibody [SP211], prediluted (ab228180)
    • Anti-CSF-1-R antibody [SP211] - BSA and Azide free (ab240265)
    • Alexa Fluor® 488 Anti-CSF-1-R antibody [SP211] (ab267541)
    • Alexa Fluor® 647 Anti-CSF-1-R antibody [SP211] (ab267542)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.
IHC-P (3)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Notes
Flow Cyt (Intra)
1/20.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function

    Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
  • Tissue specificity

    Expressed in bone marrow and in differentiated blood mononuclear cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P07333 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1436 Human
    • Entrez Gene: 12978 Mouse
    • Entrez Gene: 307403 Rat
    • Omim: 164770 Human
    • SwissProt: P07333 Human
    • SwissProt: P09581 Mouse
    • SwissProt: Q00495 Rat
    • Unigene: 586219 Human
    • Unigene: 22574 Mouse
    • Unigene: 72599 Rat
    see all
  • Alternative names

    • C FMS antibody
    • CD 115 antibody
    • CD115 antibody
    • CD115 antigen antibody
    • CFMS antibody
    • Colony stimulating factor 1 receptor antibody
    • Colony stimulating factor I receptor antibody
    • CSF 1 R antibody
    • CSF 1R antibody
    • CSF-1 receptor antibody
    • CSF-1-R antibody
    • CSF1 R antibody
    • CSF1R antibody
    • CSF1R_HUMAN antibody
    • CSFR antibody
    • EC 2.7.10.1 antibody
    • FIM 2 antibody
    • FIM2 antibody
    • FMS antibody
    • FMS proto oncogene antibody
    • FMS protooncogene antibody
    • HDLS antibody
    • M-CSF Receptor antibody
    • M-CSF-R antibody
    • Macrophage colony stimulating factor 1 receptor antibody
    • Macrophage colony stimulating factor I receptor antibody
    • Macrophage colony-stimulating factor 1 receptor antibody
    • McDonough feline sarcoma viral (v fms) oncogene homolog antibody
    • MCSFR antibody
    • Oncogen FMS antibody
    • Proto-oncogene c-Fms antibody
    • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling CSF-1-R with ab183316 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183316 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CSF-1-R with ab183316 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183316 for 10 mins at room temperature.

  • Flow Cytometry (Intracellular) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Flow Cytometry (Intracellular) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Intracellular Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma) labeling CSF-1-R with purified ab183316 at 1/20 dilution (5.65µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemical analysis of human tonsil tissue labeling CSF-1-R using ab183316 at a 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemical analysis of human skin tissue labeling CSF-1-R using ab183316 at a 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemical analysis of human skin squamous cell carcinoma tissue labeling CSF-1-R using ab183316 at a 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemical analysis of human colon tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemical analysis of human lung tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [SP211] (ab183316)

    Immunohistochemical analysis of human liver tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.

  • Anti-CSF-1-R antibody [SP211] (ab183316)
    Anti-CSF-1-R antibody [SP211] (ab183316)

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab183316? Please let us know so that we can cite the reference in this datasheet.

ab183316 has been referenced in 10 publications.

  • Tang F  et al. Patient-Derived Tumor Xenografts Plus Ex Vivo Models Enable Drug Validation for Tenosynovial Giant Cell Tumors. Ann Surg Oncol N/A:N/A (2021). PubMed: 33748895
  • Shi X  et al. Characterization of a p53/miR-34a/CSF1R/STAT3 Feedback Loop in Colorectal Cancer. Cell Mol Gastroenterol Hepatol 10:391-418 (2020). PubMed: 32304779
  • Cassetta L  et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell 35:588-602.e10 (2019). PubMed: 30930117
  • Kitadate Y  et al. Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche. Cell Stem Cell 24:79-92.e6 (2019). PubMed: 30581080
  • Rashid R  et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). PubMed: 31848351
  • Weber M  et al. Differences in Inflammation and Bone Resorption between Apical Granulomas, Radicular Cysts, and Dentigerous Cysts. J Endod 45:1200-1208 (2019). PubMed: 31400944
  • Gurevich DB  et al. Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression. EMBO J 37:N/A (2018). PubMed: 29866703
  • Komohara Y  et al. Potential anti-lymphoma effect of M-CSFR inhibitor in adult T-cell leukemia/lymphoma. J Clin Exp Hematop 58:152-160 (2018). PubMed: 30541986
  • Olmeda D  et al. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine. Nature 546:676-680 (2017). PubMed: 28658220
  • Guo X  et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). IHC, ICC/IF ; Human . PubMed: 27602954

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1-4 of 4 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CSF-1-R antibody [SP211]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (tibia)
Antigen retrieval step
Other
Permeabilization
No
Specification
tibia
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 29 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CSF-1-R antibody [SP211]

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Na-citrate, pH6.0
Permeabilization
No
Specification
Bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 23 2015

Immunohistochemistry (Frozen sections) abreview for Anti-CSF-1-R antibody [SP211]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Lymph node)
Permeabilization
No
Specification
Lymph node
Blocking step
Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Sep 15 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CSF-1-R antibody [SP211]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.0
Permeabilization
No
Specification
Lung
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 23 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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