Key features and details
- Rabbit polyclonal to CSF-1-R (phospho Y561)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CSF-1-R (phospho Y561) antibody
See all CSF-1-R primary antibodies
DescriptionRabbit polyclonal to CSF-1-R (phospho Y561)
ab192810 detects endogenous levels of CSF-1-R only when phosphorylated at tyrosine 561.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human CSF-1-R (phospho Y561) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of tyrosine 561 (N-S-Y(p)-T-F). NP_001275634.1.
Database link: P07333
- WB: HepG2 cell extracts treated with PMA. IHC-P: Human brain tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192810 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionProtein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
Tissue specificityExpressed in bone marrow and in differentiated blood mononuclear cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
- C FMS antibody
- CD 115 antibody
- CD115 antibody
All lanes : Anti-CSF-1-R (phospho Y561) antibody (ab192810) at 1/500 dilution
Lane 1 : HepG2 cell extracts treated with PMA
Lane 2 : HepG2 cell extracts treated with PMA with antigen specific peptide
Predicted band size: 108 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of formalin fixed paraffin embedded Human brain tissue labeling CSF-1-R with ab192910 at 1/50 dilution. The image on the right was pre-incubated with blocking peptide.
ab192810 has not yet been referenced specifically in any publications.