Product nameAnti-CSGALNACT1 antibody
DescriptionRabbit polyclonal to CSGALNACT1
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within internal sequence amino acids 483-532 (DELTPEQYKM CMQSKAMNEA SHGQLGMLVF RHEIEAHLRK QKQKTSSKKT) of Human CSGALNACT1 (NP_060841).
- MCF7 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
FunctionTransfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage.
Tissue specificityUbiquitous, with the highest levels in placenta, thyroid, bladder, prostate and adrenal gland. Detected at low levels in the other tissues examined.
Sequence similaritiesBelongs to the chondroitin N-acetylgalactosaminyltransferase family.
Cellular localizationGolgi apparatus > Golgi stack membrane.
- Information by UniProt
- 4-N-acetylgalactosaminyltransferase 1 antibody
- Beta4GalNAcT 1 antibody
- beta4GalNAcT antibody
Anti-CSGALNACT1 antibody (ab83071) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
This product has been referenced in:
- Sakai D et al. Upregulation of glycosaminoglycan synthesis by Neurotropin in nucleus pulposus cells via stimulation of chondroitin sulfate N-acetylgalactosaminyltransferase 1: A new approach to attenuation of intervertebral disc degeneration. PLoS One 13:e0202640 (2018). Read more (PubMed: 30148873) »