Overview

  • Product name
    Anti-CSK antibody
    See all CSK primary antibodies
  • Description
    Rabbit polyclonal to CSK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human CSK aa 375-425. The exact sequence is proprietary. (NP_004374.1).
    Sequence:

    LLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWH L


    Database link: P41240

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab186050 was affinity purified using an epitope specific to CSK immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    Images

    • Immunoprecipitation - Anti-CSK antibody (ab186050)
      Immunoprecipitation - Anti-CSK antibody (ab186050)

      Detection of CSK in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186050 at 6 µg/mg lysate for IP (Lane 1). Lane 2 represents rabbit IgG IP control. An alternative anti-CSK antibody was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab186050 has not yet been referenced specifically in any publications.

    Publishing research using ab186050? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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