Anti-CSK antibody (ab186050)

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Immunoprecipitation - Anti-CSK antibody (ab186050)

    Key features and details

    • Rabbit polyclonal to CSK
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-CSK antibody
      See all CSK primary antibodies

    • Description

      Rabbit polyclonal to CSK

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IPmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

    • Immunogen

      Synthetic peptide within Human CSK aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_004374.1).
      Database link: P41240

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Jurkat whole cell lysate (ab7899).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: 99% Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab186050 was affinity purified using an epitope specific to CSK immobilized on solid support.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab186050 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at 2-10 µg/mg of lysate.
    Notes
    IP
    Use at 2-10 µg/mg of lysate.

    Target

    Images

    • Immunoprecipitation - Anti-CSK antibody (ab186050)
      Immunoprecipitation - Anti-CSK antibody (ab186050)

      Detection of CSK in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186050 at 6 µg/mg lysate for IP (Lane 1). Lane 2 represents rabbit IgG IP control. An alternative anti-CSK antibody was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References (0)

    Publishing research using ab186050? Please let us know so that we can cite the reference in this datasheet.

    ab186050 has not yet been referenced specifically in any publications.

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