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  1. Link

    csk-antibody-ab186050.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Src Family
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Anti-CSK antibody (ab186050)

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Immunoprecipitation - Anti-CSK antibody (ab186050)

    Key features and details

    • Rabbit polyclonal to CSK
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CSK antibody
      See all CSK primary antibodies
    • Description

      Rabbit polyclonal to CSK
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
    • Immunogen

      Synthetic peptide within Human CSK aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_004374.1).
      Database link: P41240

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Jurkat whole cell lysate (ab7899).
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: 99% Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab186050 was affinity purified using an epitope specific to CSK immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Src Family
      • Neuroscience
      • Development

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat whole cell lysate (ab30128)
      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant human CSK protein (ab60764)
    • Related Products

      • Recombinant human CSK protein (ab60764)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab186050 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at 2-10 µg/mg of lysate.
    Notes
    IP
    Use at 2-10 µg/mg of lysate.

    Target

    • Function

      Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
    • Tissue specificity

      Expressed in lung and macrophages.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications

      Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
    • Cellular localization

      Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
    • Target information above from: UniProt accession P41240 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396396 Chicken
      • Entrez Gene: 453745 Chimpanzee
      • Entrez Gene: 509246 Cow
      • Entrez Gene: 487649 Dog
      • Entrez Gene: 1445 Human
      • Entrez Gene: 12988 Mouse
      • Entrez Gene: 100154110 Pig
      • Entrez Gene: 315707 Rat
      • Entrez Gene: 702868 Rhesus monkey
      • Omim: 124095 Human
      • SwissProt: P41239 Chicken
      • SwissProt: Q0VBZ0 Cow
      • SwissProt: P41240 Human
      • SwissProt: P41241 Mouse
      • SwissProt: P32577 Rat
      • Unigene: 77793 Human
      • Unigene: 21974 Mouse
      • Unigene: 470150 Mouse
      • Unigene: 2759 Rat
      see all
    • Alternative names

      • C SRC antibody
      • C SRC kinase antibody
      • C src Tyrosine Kinase antibody
      • C-SRC kinase antibody
      • c-src tyrosine kinase antibody
      • Csk A antibody
      • CSK antibody
      • CSK_HUMAN antibody
      • CYTOPLASMIC TYROSINE KINASE antibody
      • EC 2.7.10.2 antibody
      • MGC112926 antibody
      • MGC117393 antibody
      • MGC154049 antibody
      • P60 Src antibody
      • Protein tyrosine kinase CYL antibody
      • Protein-tyrosine kinase CYL antibody
      • Proto oncogene tyrosine protein kinase antibody
      • Tyrosine protein kinase CSK antibody
      • Tyrosine-protein kinase CSK antibody
      • zgc:154049 antibody
      see all

    Images

    • Immunoprecipitation - Anti-CSK antibody (ab186050)
      Immunoprecipitation - Anti-CSK antibody (ab186050)

      Detection of CSK in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186050 at 6 µg/mg lysate for IP (Lane 1). Lane 2 represents rabbit IgG IP control. An alternative anti-CSK antibody was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

    Protocols

    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab186050? Please let us know so that we can cite the reference in this datasheet.

    ab186050 has not yet been referenced specifically in any publications.

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