Key features and details
- Goat polyclonal to CSK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CSK antibody
See all CSK primary antibodies
DescriptionGoat polyclonal to CSK
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog
- Jurkat cell lysate, Human colon tissue.
General notesGenBank Accession Number – NP_004374.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use at an assay dependent concentration.|
FunctionSpecifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Tissue specificityExpressed in lung and macrophages.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsAutophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cellular localizationCytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
- Information by UniProt
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
ab744 staining (0.5
µg/ml) of Jurkat lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
ab744 has been referenced in 2 publications.
- Yeh WL et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . PubMed: 23593342
- Ribeiro-Oliveira A et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . PubMed: 18701577