Key features and details
- Rabbit polyclonal to CSK - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CSK antibody - C-terminal
See all CSK primary antibodies
DescriptionRabbit polyclonal to CSK - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
FunctionSpecifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Tissue specificityExpressed in lung and macrophages.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsAutophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cellular localizationCytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
- Information by UniProt
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
All lanes : Anti-CSK antibody - C-terminal (ab186069) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : Mouse TCMK-1 whole cell lysate
Lane 4 : Mouse NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes
Immunoprecipitation of CSK from Jurkat whole cell lysate by ab186069 at 1 µg/mg lysate (lane 1). 1 mg of Jurkat whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control. Detection: chemiluminescence. Exposure time: 10 seconds.
ab186069 has not yet been referenced specifically in any publications.