Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6771] to CSK
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-CSK antibody [EPR6771]
See all CSK primary antibodies
DescriptionRabbit monoclonal [EPR6771] to CSK
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CSK aa 50-150. The exact sequence is proprietary.
- Jurkat, Ramos and RAW 264.7 cell lysates; Human tonsil tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
FunctionSpecifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Tissue specificityExpressed in lung and macrophages.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsAutophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cellular localizationCytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
- Information by UniProt
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CSK knockout HAP1 cell lysate (20 µg)
Lane 3: Ramos cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab125005 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab125005 was shown to specifically react with CSK when CSK knockout samples were used. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab125005 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-CSK antibody [EPR6771] (ab125005) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
ab125005 at 1/50 dilution staining CSK in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab125005 has been referenced in 1 publication.
- Shields DJ et al. Oncogenic Ras/Src cooperativity in pancreatic neoplasia. Oncogene 30:2123-34 (2011). PubMed: 21242978