• Product name

    Anti-CSK antibody [EPR6771]
    See all CSK primary antibodies
  • Description

    Rabbit monoclonal [EPR6771] to CSK
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CSK aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Jurkat, Ramos and RAW 264.7 cell lysates; Human tonsil tissue.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 5.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab125005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
    • Tissue specificity

      Expressed in lung and macrophages.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Post-translational

      Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
    • Cellular localization

      Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
    • Information by UniProt
    • Database links

    • Alternative names

      • C SRC antibody
      • C SRC kinase antibody
      • C src Tyrosine Kinase antibody
      • C-SRC kinase antibody
      • c-src tyrosine kinase antibody
      • Csk A antibody
      • CSK antibody
      • CSK_HUMAN antibody
      • EC antibody
      • MGC112926 antibody
      • MGC117393 antibody
      • MGC154049 antibody
      • P60 Src antibody
      • Protein tyrosine kinase CYL antibody
      • Protein-tyrosine kinase CYL antibody
      • Proto oncogene tyrosine protein kinase antibody
      • Tyrosine protein kinase CSK antibody
      • Tyrosine-protein kinase CSK antibody
      • zgc:154049 antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CSK knockout HAP1 cell lysate (20 µg)
      Lane 3: Ramos cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab125005 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab125005 was shown to specifically react with CSK when CSK knockout samples were used. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab125005 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-CSK antibody [EPR6771] (ab125005) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : RAW 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 50 kDa

    • ab125005 at 1/50 dilution staining CSK in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    ab125005 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Thank you for contacting us.
    ab104839 was generated against amino acids x-y.

    ab93129 was generated against a partial recombinant protein containing the protein sequence spanning 163-514. A his-tag was used in the protein.

    ab92379 The peptide maps between amino acids 40 and 65 of human ROR2 (UniProt: Q01974), i.e. an extracellular epitope is detected.
    ab80174 The antigen is recorded in our system as a his-tag fusion protein, without specifying whether it is a partial or full-length recombinant protein. We apologise that more information is currently not available.

    ab81599 was generated against amino acids 2-100.
    ab92302 The peptide maps between amino acids 25 and 50 of human FGR (UniProt: P09769)
    ab65007 The amino acid range for this immunogen is 61-110 from human FGR (P09769).

    ab109740 The recombinant protein used as the immunogen for this antibody encodes amino acids x-y.
    ab52273 was generated against amino acids 2-175.

    ab108418 The peptide maps between amino acids 760 and 790 of human FES (UniProt: P07332).

    ab91623 was generated against amino acids x-y.

    ab119855 was generated against amino acids 7-176

    ab125005 The peptide maps between amino acids 75 and 105 of human CSK (UniProt: P41240)

    ab52563 see attachment

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up