Recombinant Anti-CSL4 antibody [EPR13526] (ab181108)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13526] to CSL4
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CSL4 antibody [EPR13526]
See all CSL4 primary antibodies -
Description
Rabbit monoclonal [EPR13526] to CSL4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji, 293, K562, C6, Raw264.7, PC-12 and NIH/3T3 cell lysate. Human cervix carcinoma and colon tissue. 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13526 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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IP |
1/60.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
IP
1/60. |
Target
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Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC1 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8. -
Sequence similarities
Contains 1 S1 motif domain. -
Cellular localization
Nucleus > nucleolus. Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 51013 Human
- Entrez Gene: 66583 Mouse
- Entrez Gene: 679140 Rat
- Omim: 606493 Human
- SwissProt: Q9Y3B2 Human
- SwissProt: Q9DAA6 Mouse
- Unigene: 632089 Human
- Unigene: 289086 Mouse
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Alternative names
- 3'-5' exoribonuclease CSL4 homolog antibody
- CGI 108 antibody
- CGI-108 antibody
see all
Images
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All lanes : Anti-CSL4 antibody [EPR13526] (ab181108) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EXOSC1 CRISPR-Cas9 edited HeLa cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CSL4 antibody [EPR13526] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181108 was shown to bind specifically to CSL4. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in EXOSC1 CRISPR-Cas9 edited cell line ab265194 (EXOSC1 CRISPR-Cas9 edited cell lysate ab257945). The band observed in the CRISPR-Cas9 edited lysate lane above 22 kDa is likely to represent CSL4 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and EXOSC1 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-CSL4 antibody [EPR13526] (ab181108) at 1/2000 dilution
Lane 1 : K562 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Raw264.7 cell lysate
Lane 4 : PC-12 cell lysate
Lane 5 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Western blot analysis of CSL4 in immunoprecipitation pellets from Raji lysate. ab181108 used at 1/60 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Lane 2 shows negative control.
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Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling CSL4 with ab181108 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488), Conterstained with DAPI blue.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-CSL4 antibody [EPR13526] (ab181108) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181108 has not yet been referenced specifically in any publications.