Overview

  • Product name

    Anti-CSL4 antibody [EPR13526]
    See all CSL4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13526] to CSL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human CSL4 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y3B2

  • Positive control

    • Raji, 293, K562, C6, Raw264.7, PC-12 and NIH/3T3 cell lysate. Human cervix carcinoma and colon tissue. 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13526
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/60.

Target

  • Function

    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC1 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8.
  • Sequence similarities

    Contains 1 S1 motif domain.
  • Cellular localization

    Nucleus > nucleolus. Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3'-5' exoribonuclease CSL4 homolog antibody
    • CGI 108 antibody
    • CGI-108 antibody
    • CSL4 antibody
    • CSL4 exosomal core protein homolog antibody
    • CSL4, S. cerevisiae, homolog of antibody
    • Csl4p antibody
    • EXOS1_HUMAN antibody
    • Exosc1 antibody
    • Exosomal core protein CSL4 antibody
    • Exosome complex component csl4 antibody
    • Exosome component 1 antibody
    • hCsl4p antibody
    • Homolog of yeast exosomal core protein CSL4 antibody
    • p13 antibody
    • RP11-452K12.9 antibody
    • SKI4 antibody
    • Ski4p antibody
    see all

Images

  • All lanes : Anti-CSL4 antibody [EPR13526] (ab181108) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : Raw264.7 cell lysate
    Lane 4 : PC-12 cell lysate
    Lane 5 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.

  • Western blot analysis of CSL4 in immunoprecipitation pellets from Raji lysate. ab181108 used at 1/60 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Lane 2 shows negative control.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling CSL4 with ab181108 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488), Conterstained with DAPI blue.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.

  • All lanes : Anti-CSL4 antibody [EPR13526] (ab181108) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

References

ab181108 has not yet been referenced specifically in any publications.

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