Anti-CSN1 antibody (ab10413)

Submit a review Q&A (1)References (2)

Overview

  • Product name
    Anti-CSN1 antibody
    See all CSN1 primary antibodies
  • Description
    Rabbit polyclonal to CSN1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human COP9 signalosome complex subunit 1 encoded within exons 13 and 14 (LocusLink ID 2873).

  • Positive control
    • Whole cell lysate from mock transfected or CSN1 transfected HEK293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab10413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the CSN1 family.
    Contains 1 PCI domain.
  • Domain
    The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
    The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • COP9 signalosome complex subunit 1 antibody
    • COP9 signalosome subunit 1 antibody
    • COPS1 antibody
    • CSN 1 antibody
    • CSN1_HUMAN antibody
    • FUS6/COP11, Arabidopsis, homolog of antibody
    • G protein pathway suppressor 1 antibody
    • GPS-1 antibody
    • Gps1 antibody
    • JAB1-containing signalosome subunit 1 antibody
    • Protein GPS1 antibody
    • Protein MFH antibody
    • SGN1 antibody
    • Signalosome subunit 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (ab10413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (ab10413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSN1 with ab10413 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot - Anti-CSN1 antibody (ab10413)
    Western blot - Anti-CSN1 antibody (ab10413)
    Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 mcg – E; 10 mcg – T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells.

References

This product has been referenced in:
  • Pesaresi M  et al. Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features. Biol Sex Differ 9:6 (2018). Read more (PubMed: 29351809) »
  • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). Read more (PubMed: 16624822) »
See all 2 Publications for this product

Publishing research using ab10413? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I was looking up the CSN1 antibody you described has been synthesized according to the exon sequence between exon 13 and 14 of the respective human gene. Now we would like to use this antibody against an ortholog in Aspergillus nidulans a filamentous fungus. Since my experience with antibodies is limited I would greatly appreciate if you could evaluate our chances on how good this antibody would work against our protein. To give you some more information, this is the genbank accession number where you can find the information about coding sequences and predicted amino acid sequences: AAT73769 Also it would be of interest as to where the antibody would most likely bind, since I was unable to follow the locus link ID you provided, an indication showing us the involved amino acid region would be very helpful. Thank you very much for your support. With kind regard Oliver Draht

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Answer

Thank you for your enquiry. This product will not work for E. nidulans. The epitope recognized by ab10413 maps to a region between residue 400 and the C-terminus (residue 471) of human G protein Pathway Suppressor 1 using the numbering given in Swiss-Prot entry Q13098 (GeneID 2873). I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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