Anti-CSN1 antibody (ab10413)

Submit a review Q&A (1)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (ab10413)
  • Western blot - Anti-CSN1 antibody (ab10413)

Key features and details

  • Rabbit polyclonal to CSN1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CSN1 antibody
    See all CSN1 primary antibodies

  • Description

    Rabbit polyclonal to CSN1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human COP9 signalosome complex subunit 1 encoded within exons 13 and 14 (LocusLink ID 2873).

  • Positive control

    • Whole cell lysate from mock transfected or CSN1 transfected HEK293T cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Horse
Chicken
Guinea pig
Cow
Dog
Turkey
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction.

  • Tissue specificity

    Widely expressed.

  • Sequence similarities

    Belongs to the CSN1 family.
    Contains 1 PCI domain.

  • Domain

    The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
    The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • COP9 signalosome complex subunit 1 antibody
    • COP9 signalosome subunit 1 antibody
    • COPS1 antibody
    • CSN 1 antibody
    • CSN1_HUMAN antibody
    • FUS6/COP11, Arabidopsis, homolog of antibody
    • G protein pathway suppressor 1 antibody
    • GPS-1 antibody
    • Gps1 antibody
    • JAB1-containing signalosome subunit 1 antibody
    • Protein GPS1 antibody
    • Protein MFH antibody
    • SGN1 antibody
    • Signalosome subunit 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (ab10413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (ab10413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSN1 with ab10413 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot - Anti-CSN1 antibody (ab10413)
    Western blot - Anti-CSN1 antibody (ab10413)
    Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 mcg – E; 10 mcg – T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells.

References (3)

Publishing research using ab10413? Please let us know so that we can cite the reference in this datasheet.

ab10413 has been referenced in 3 publications.

  • Nara K  et al. Anti-inflammatory roles of microRNA 21 in lipopolysaccharide-stimulated human dental pulp cells. J Cell Physiol N/A:N/A (2019). PubMed: 31042008
  • Pesaresi M  et al. Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features. Biol Sex Differ 9:6 (2018). PubMed: 29351809
  • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822

Customer reviews and Q&As

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Question

I was looking up the CSN1 antibody you described has been synthesized according to the exon sequence between exon 13 and 14 of the respective human gene. Now we would like to use this antibody against an ortholog in Aspergillus nidulans a filamentous fungus. Since my experience with antibodies is limited I would greatly appreciate if you could evaluate our chances on how good this antibody would work against our protein. To give you some more information, this is the genbank accession number where you can find the information about coding sequences and predicted amino acid sequences: AAT73769 Also it would be of interest as to where the antibody would most likely bind, since I was unable to follow the locus link ID you provided, an indication showing us the involved amino acid region would be very helpful. Thank you very much for your support. With kind regard Oliver Draht

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Answer

Thank you for your enquiry. This product will not work for E. nidulans. The epitope recognized by ab10413 maps to a region between residue 400 and the C-terminus (residue 471) of human G protein Pathway Suppressor 1 using the numbering given in Swiss-Prot entry Q13098 (GeneID 2873). I hope this information helps. Please do not hesitate to contact us if you need anything further.

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