RabMAb

Anti-CSN1 antibody [EP15642-22] (ab194359)

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Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)
  • Immunocytochemistry/ Immunofluorescence - Anti-CSN1 antibody [EP15642-22] (ab194359)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)
  • Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)

Key features and details

  • Rabbit monoclonal [EP15642-22] to CSN1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CSN1 antibody [EP15642-22]
    See all CSN1 primary antibodies

  • Description

    Rabbit monoclonal [EP15642-22] to CSN1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CSN1 aa 50-200. The exact sequence is proprietary.
    Database link: Q13098

  • Positive control

    • HeLa, K562, MCF7 and 293T cell lysates; Mouse brain, kidney, heart and spleen tissue lysates; Rat brain, kidney, heart and spleen tissue lysates; Human bladder transitional cell carcinoma and mouse kidney tissues; HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab194359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/150 - 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/150.

Target

  • Function

    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction.

  • Tissue specificity

    Widely expressed.

  • Sequence similarities

    Belongs to the CSN1 family.
    Contains 1 PCI domain.

  • Domain

    The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
    The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • COP9 signalosome complex subunit 1 antibody
    • COP9 signalosome subunit 1 antibody
    • COPS1 antibody
    • CSN 1 antibody
    • CSN1_HUMAN antibody
    • FUS6/COP11, Arabidopsis, homolog of antibody
    • G protein pathway suppressor 1 antibody
    • GPS-1 antibody
    • Gps1 antibody
    • JAB1-containing signalosome subunit 1 antibody
    • Protein GPS1 antibody
    • Protein MFH antibody
    • SGN1 antibody
    • Signalosome subunit 1 antibody
    see all

Images

  • Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)
    Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)
    All lanes : Anti-CSN1 antibody [EP15642-22] (ab194359) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CSN1 antibody [EP15642-22] (ab194359)
    Immunocytochemistry/ Immunofluorescence - Anti-CSN1 antibody [EP15642-22] (ab194359)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CSN1 with ab194359 at 1/150 dilution (12 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CSN1 with ab194359 at 1/300 (6μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution (ab97051) and counter-stained with Hematoxylin. (inset: negative control).

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)

    Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CSN1 with ab194359 at 1/300 (6μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution  (ab97051) and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)
    Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)
    All lanes : Anti-CSN1 antibody [EP15642-22] (ab194359) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa

References (0)

Publishing research using ab194359? Please let us know so that we can cite the reference in this datasheet.

ab194359 has not yet been referenced specifically in any publications.

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