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    csn2-antibody-ab10426.pdf

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Neuroscience Neurotransmission Intracellular Signaling Kinases
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Anti-CSN2 antibody (ab10426)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Western blot - Anti-CSN2 antibody (ab10426)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN2 antibody (ab10426)

Key features and details

  • Rabbit polyclonal to CSN2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CSN2 antibody
    See all CSN2 primary antibodies
  • Description

    Rabbit polyclonal to CSN2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to CSN2. The epitope recognized by ab10426 maps to a region between residues 1 and 50 of human CSN2.
    Database link: P61201

  • Positive control

    • Purchase matching WB positive control:Recombinant CSN2 protein
    • Whole cell lysate from mock transfected or CSN2 transfected HEK293T cells. IHC human controls: Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer IHC mouse controls: Squamous Cell Carcinoma
  • General notes


    CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.

     This product was previously labelled as TRIP15

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were affinity purified using the peptide immobilized on solid support.
  • Primary antibody notes

    CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CSN2 protein (His tag) (ab241234)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Rat
Rabbit
Horse
Chicken
Guinea pig
Cow
Dog
Pig
Xenopus laevis
Chimpanzee
Drosophila melanogaster
Zebrafish
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
  • Sequence similarities

    Belongs to the CSN2 family.
    Contains 1 PCI domain.
  • Post-translational
    modifications

    Phosphorylated by CK2 and PKD kinases.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P61201 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 430917 Chicken
    • Entrez Gene: 34225 Drosophila melanogaster
    • Entrez Gene: 9318 Human
    • Entrez Gene: 100125956 Pig
    • Entrez Gene: 261736 Rat
    • Entrez Gene: 548537 Xenopus tropicalis
    • Entrez Gene: 415145 Zebrafish
    • Omim: 604508 Human
    • SwissProt: Q94899 Drosophila melanogaster
    • SwissProt: P61201 Human
    • SwissProt: P61203 Rat
    • SwissProt: Q6IR75 Xenopus laevis
    • SwissProt: Q6IQT4 Zebrafish
    • Unigene: 20642 Drosophila melanogaster
    • Unigene: 369614 Human
    • Unigene: 11556 Rat
    • Unigene: 83155 Xenopus laevis
    • Unigene: 78251 Zebrafish
    see all
  • Alternative names

    • ALIEN antibody
    • Alien Drosophila himolog of antibody
    • Alien homolog antibody
    • COP 9 antibody
    • COP9 antibody
    • COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) antibody
    • COP9 constitutive photomorphogenic homolog subunit 2 antibody
    • COP9 signalosome complex subunit 2 antibody
    • COPS 2 antibody
    • Cops2 antibody
    • CSN2 antibody
    • CSN2_HUMAN antibody
    • JAB1 containing signalosome subunit 2 antibody
    • JAB1-containing signalosome subunit 2 antibody
    • SGN 2 antibody
    • SGN2 antibody
    • Signalosome subunit 2 antibody
    • Thyroid Hormone Receptor Interactor15 antibody
    • Thyroid Hormone Receptor Interactor 15 antibody
    • Thyroid receptor-interacting protein 15 antibody
    • TR-interacting protein 15 antibody
    • TRIP 15 antibody
    • TRIP-15 antibody
    • TRIP15 antibody
    see all

Images

  • Western blot - Anti-CSN2 antibody (ab10426)
    Western blot - Anti-CSN2 antibody (ab10426)

    Whole cell lysate (50 μg - E; 10 μg - T) from mock transfected (E) or CSN2 transfected (T) HEK293T cells. Antibody used at the indicated concentration.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN2 antibody (ab10426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN2 antibody (ab10426)

    IHC-P section of human prostate carcinoma (left) and mouse squamous cell carcinoma (right).

    Antibody used at a dilution of 1:5,000 (0.2μg/ml) and 1:1,000 (1μg/ml).

    Detection: DAB

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab10426? Please let us know so that we can cite the reference in this datasheet.

    ab10426 has been referenced in 1 publication.

    • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    We ordered two samples of anti-CSN2 (ab10426) last May. Our Purchase Order number was CAMPANERO-0161. It seems there was some stock shortage and we received the second sample 1-2 months after the first one. This is likely the reason because these two samples were from different lots (lots numbers 114133 and 82446). We obviously started by using the first lot that we received (114133) and noticed that it worked out well in immunoblotting experiments designed to detect the endogenous protein in samples derived from human cell lines as well as in experiments designed to detect ectopically expressed CSN2. As this lot was getting finished, we started to use the second lot and found no specific signals. At the beginning we thought we were doing something wrong, but now we designed an experiment with the only purpose to compare both lots and our results clearly indicate that this second lot (82446) is not working at all. We have carried out two different experiments to compare both lots performance. In the first experiment (see enclosed file with Fig 1a and 1b), we immunoblotted extracts from cells transfected with either a mock vector or with a vector encoding a GST fusion with either CSN2 alpha or CSN2 beta. This nitrocellulose membrane was probed first with anti-CSN2 (lot 114133) and second with anti-GST. As you can see, the anti-CSN2 antibody (lot 114133) reacts with both the GST fusion proteins and the endogenous CSN2 alpha (Alien alpha) while the anti-GST Ab only recognizes the GST fusion proteins. In the second experiment we transfected Saos-2 cells with different expression vectors for CSN2 (Alien) alpha or beta tagged with either HA or GST. Please, note that pMT2-driven Alien alpha is expected to be larger than the pcDNA3-driven version. Cell extracts were immunoblotted and the membrane was sequentially probed with anti-CSN2 (lot 82446), anti-HA, and anti-GST. As you can see in Fig. 2 (see enclosed file), the anti-alien Ab was unable to react with any of the different transfected CSN2 forms at all, while the anti-HA recognized the HA-tagged versions of Alien alpha and beta and the anti-GST Ab reacted with the GST-tagged CSN2 alpha and beta isoforms. Please, notice that we used the same extracts for lanes corresponding to GST-Alien alpha or GST-Alien beta that we used in Fig 1. We believe these results clearly demonstrate that anti-CSN2 ab10426 (lot 82446) fails to work as expected. Unfortunately, we have lost a lot of time and wasted a lot of reagents by using this antibody and we would like, at least, to receive a sample that works fine from a different lot. Finally, according to antibody ab10426 datasheet, this antibody is predicted to react with Mouse due to sequence homology. In this regard, we wanted to let you know that, at least in our hands, this antibody fails to recognize the mouse CSN2 protein and we wonder if you have any new information about tests performed with this or with any other anti-CSN2 antibody on mouse proteins.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2006

    Answer

    Thank you for your enquiry. May I also thank you for explaining the problem so well. The images that you e-mailed are beautiful and clearly highlight the problems that you have been having. We often observe LOT variation in our antibodies. This can be attributable to the preparation of the antibody. From the results that you have shown me I can clearly see that there is an element of LOT variation. Whilst you are detecting both the endogenous CSN2 (Alien) and ectopically expressed GST tagged forms of the protein with LOT number 114133 you are only detecting the endogenous forms of the protein with LOT 82446. Quality is important to Abcam and I thank you once again for highlighting this to us. Unfortunately I cannot provide you with a replacement vial as your purchase falls outside of our 90 day refund and credit note policy. We currently have two lots in stock 114133 and 114751. Were you wishing to place an order you are welcome to specifically request LOT 114133 given that this has behaved well in your experiments. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 01 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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