Anti-CSN2 antibody (ab10426)
Key features and details
- Rabbit polyclonal to CSN2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSN2 antibody
See all CSN2 primary antibodies -
Description
Rabbit polyclonal to CSN2 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to CSN2. The epitope recognized by ab10426 maps to a region between residues 1 and 50 of human CSN2.
Database link: P61201 -
Positive control
- Purchase matching WB positive control:Recombinant CSN2 protein
- Whole cell lysate from mock transfected or CSN2 transfected HEK293T cells. IHC human controls: Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer IHC mouse controls: Squamous Cell Carcinoma
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General notes
CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.
This product was previously labelled as TRIP15
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Primary antibody notes
CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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All applications |
Rat
Rabbit
Horse
Chicken
Guinea pig
Cow
Dog
Pig
Xenopus laevis
Chimpanzee
Drosophila melanogaster
Zebrafish
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1. -
Sequence similarities
Belongs to the CSN2 family.
Contains 1 PCI domain. -
Post-translational
modificationsPhosphorylated by CK2 and PKD kinases. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 430917 Chicken
- Entrez Gene: 34225 Drosophila melanogaster
- Entrez Gene: 9318 Human
- Entrez Gene: 100125956 Pig
- Entrez Gene: 261736 Rat
- Entrez Gene: 548537 Xenopus tropicalis
- Entrez Gene: 415145 Zebrafish
- Omim: 604508 Human
see all -
Alternative names
- ALIEN antibody
- Alien Drosophila himolog of antibody
- Alien homolog antibody
see all
Images
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Whole cell lysate (50 μg - E; 10 μg - T) from mock transfected (E) or CSN2 transfected (T) HEK293T cells. Antibody used at the indicated concentration.
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IHC-P section of human prostate carcinoma (left) and mouse squamous cell carcinoma (right).
Antibody used at a dilution of 1:5,000 (0.2μg/ml) and 1:1,000 (1μg/ml).
Detection: DAB
Protocols
References (1)
ab10426 has been referenced in 1 publication.
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822