Product nameAnti-CSN2 antibody
See all CSN2 primary antibodies
DescriptionRabbit polyclonal to CSN2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to CSN2. The epitope recognized by ab10426 maps to a region between residues 1 and 50 of human CSN2.
Database link: P61201
- Purchase matching WB positive control:Recombinant CSN2 protein
- Whole cell lysate from mock transfected or CSN2 transfected HEK293T cells. IHC human controls: Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer IHC mouse controls: Squamous Cell Carcinoma
CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.
This product was previously labelled as TRIP15
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
Primary antibody notesCSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab10426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
Sequence similaritiesBelongs to the CSN2 family.
Contains 1 PCI domain.
modificationsPhosphorylated by CK2 and PKD kinases.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Whole cell lysate (50 μg - E; 10 μg - T) from mock transfected (E) or CSN2 transfected (T) HEK293T cells. Antibody used at the indicated concentration.
IHC-P section of human prostate carcinoma (left) and mouse squamous cell carcinoma (right).
Antibody used at a dilution of 1:5,000 (0.2μg/ml) and 1:1,000 (1μg/ml).