Product nameAnti-CSN2 antibody
See all CSN2 primary antibodies
DescriptionRabbit polyclonal to CSN2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
Synthetic peptide corresponding to Human CSN2 aa 381-443.
Database link: P61201
- Purchase matching WB positive control:Recombinant CSN2 protein
- A431, H1299 and HeLa whole cell lysate; HepG2 cell line; HeLa cells; Human Mahlavu xenograft tissue.
This product was previously labelled as TRIP15
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionEssential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
Sequence similaritiesBelongs to the CSN2 family.
Contains 1 PCI domain.
modificationsPhosphorylated by CK2 and PKD kinases.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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All lanes : Anti-CSN2 antibody (ab155774) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CSN2 with ab155774 at 1/500 dilution. Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded Human Mahlavu xenograft tissue, labeling CSN2 with ab155774 at 1/100 dilution.