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    csn2-antibody-ab155774.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Coat Proteins
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Anti-CSN2 antibody (ab155774)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

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Western blot - Anti-CSN2 antibody (ab155774)
  • Immunocytochemistry/ Immunofluorescence - Anti-CSN2 antibody (ab155774)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN2 antibody (ab155774)

Key features and details

  • Rabbit polyclonal to CSN2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-CSN2 antibody
    See all CSN2 primary antibodies
  • Description

    Rabbit polyclonal to CSN2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human CSN2 aa 381-443.
    Database link: P61201

  • Positive control

    • Purchase matching WB positive control:Recombinant CSN2 protein
    • A431, H1299 and HeLa whole cell lysate; HepG2 cell line; HeLa cells; Human Mahlavu xenograft tissue.
  • General notes

     This product was previously labelled as TRIP15

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human CSN2 protein (His tag) (ab241234)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Chicken
Cow
Pig
Xenopus laevis
Monkey
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
  • Sequence similarities

    Belongs to the CSN2 family.
    Contains 1 PCI domain.
  • Post-translational
    modifications

    Phosphorylated by CK2 and PKD kinases.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P61201 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 430917 Chicken
    • Entrez Gene: 9318 Human
    • Entrez Gene: 12848 Mouse
    • Entrez Gene: 100125956 Pig
    • Entrez Gene: 261736 Rat
    • Entrez Gene: 548537 Xenopus tropicalis
    • Omim: 604508 Human
    • SwissProt: P61201 Human
    • SwissProt: P61202 Mouse
    • SwissProt: P61203 Rat
    • SwissProt: Q6IR75 Xenopus laevis
    • Unigene: 369614 Human
    • Unigene: 3596 Mouse
    • Unigene: 11556 Rat
    • Unigene: 83155 Xenopus laevis
    see all
  • Alternative names

    • ALIEN antibody
    • Alien Drosophila himolog of antibody
    • Alien homolog antibody
    • COP 9 antibody
    • COP9 antibody
    • COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) antibody
    • COP9 constitutive photomorphogenic homolog subunit 2 antibody
    • COP9 signalosome complex subunit 2 antibody
    • COPS 2 antibody
    • Cops2 antibody
    • CSN2 antibody
    • CSN2_HUMAN antibody
    • JAB1 containing signalosome subunit 2 antibody
    • JAB1-containing signalosome subunit 2 antibody
    • SGN 2 antibody
    • SGN2 antibody
    • Signalosome subunit 2 antibody
    • Thyroid Hormone Receptor Interactor15 antibody
    • Thyroid Hormone Receptor Interactor 15 antibody
    • Thyroid receptor-interacting protein 15 antibody
    • TR-interacting protein 15 antibody
    • TRIP 15 antibody
    • TRIP-15 antibody
    • TRIP15 antibody
    see all

Images

  • Western blot - Anti-CSN2 antibody (ab155774)
    Western blot - Anti-CSN2 antibody (ab155774)
    All lanes : Anti-CSN2 antibody (ab155774) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-CSN2 antibody (ab155774)
    Immunocytochemistry/ Immunofluorescence - Anti-CSN2 antibody (ab155774)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CSN2 with ab155774 at 1/500 dilution. Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN2 antibody (ab155774)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN2 antibody (ab155774)

    Immunohistochemical analysis of paraffin-embedded Human Mahlavu xenograft tissue, labeling CSN2 with ab155774 at 1/100 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab155774? Please let us know so that we can cite the reference in this datasheet.

    ab155774 has been referenced in 1 publication.

    • Liu J  et al. A genome-scale CRISPR-Cas9 screening in myeloma cells identifies regulators of immunomodulatory drug sensitivity. Leukemia N/A:N/A (2018). PubMed: 30026574

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