Key features and details
- Rabbit polyclonal to CSN2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CSN2 antibody
See all CSN2 primary antibodies
DescriptionRabbit polyclonal to CSN2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis, Drosophila melanogaster, Zebrafish
- IHC: Human stomach tissue. WB: HEK-293T overexpressing CSN2 cell lysate.
This product was previously labelled as TRIP15
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab223764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
Sequence similaritiesBelongs to the CSN2 family.
Contains 1 PCI domain.
modificationsPhosphorylated by CK2 and PKD kinases.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- ALIEN antibody
- Alien Drosophila himolog of antibody
- Alien homolog antibody
Paraffin-embedded human stomach tissue stained for CSN2 using ab223764 at 1/20 dilution in immunohistochemical analysis.
All lanes : Anti-CSN2 antibody (ab223764) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing CSN2 (co-expressed with a C-terminal myc-DDK tag), cell lysate
Developed using the ECL technique.
Predicted band size: 52 kDa
ab223764 has not yet been referenced specifically in any publications.