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    csn4-antibody-ab12322.pdf

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Neuroscience Neurotransmission Intracellular Signaling Kinases
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Anti-CSN4 antibody (ab12322)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Western blot - Anti-CSN4 antibody (ab12322)

    Key features and details

    • Rabbit polyclonal to CSN4
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CSN4 antibody
      See all CSN4 primary antibodies
    • Description

      Rabbit polyclonal to CSN4
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan
    • Immunogen

      Synthetic peptide within Human CSN4 aa 350 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
      Database link: Q9BT78

    • Positive control

      • WB: HeLa, HEK293T, Jurkat, TCMK-1, NIH/3T3
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified using the peptide immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Intracellular Signaling
      • Kinases
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Proteasome
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab12322 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
    Notes
    WB
    1/2000 - 1/10000. Predicted molecular weight: 49 kDa.

    Target

    • Function

      Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
    • Sequence similarities

      Belongs to the CSN4 family.
      Contains 1 PCI domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession Q9BT78 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 422594 Chicken
      • Entrez Gene: 461207 Chimpanzee
      • Entrez Gene: 540223 Cow
      • Entrez Gene: 51138 Human
      • Entrez Gene: 26891 Mouse
      • Entrez Gene: 100125960 Pig
      • Entrez Gene: 360915 Rat
      • Entrez Gene: 693435 Rhesus monkey
      • Entrez Gene: 325592 Zebrafish
      • SwissProt: Q3SZA0 Cow
      • SwissProt: Q9BT78 Human
      • SwissProt: O88544 Mouse
      • SwissProt: A7Y521 Pig
      • SwissProt: Q68FS2 Rat
      • SwissProt: Q6P0H6 Zebrafish
      • Unigene: 190384 Human
      • Unigene: 957 Mouse
      • Unigene: 7506 Rat
      • Unigene: 77245 Zebrafish
      see all
    • Alternative names

      • CH4 antibody
      • COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
      • COP9 complex homolog subunit 4 antibody
      • COP9 complex S4 antibody
      • COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis) antibody
      • COP9 constitutive photomorphogenic homolog subunit 4 antibody
      • COP9 signalosome complex subunit 4 antibody
      • COP9 signalosome subunit 4 antibody
      • COPS4 antibody
      • CSN complex subunit 4 antibody
      • CSN4 antibody
      • CSN4 SCHPO antibody
      • CSN4_HUMAN antibody
      • DCH4 antibody
      • JAB1 containing signalosome subunit 4 antibody
      • JAB1-containing signalosome subunit 4 antibody
      • MGC94896 antibody
      • SGN4 antibody
      • Signalosome subunit 4 antibody
      see all

    Images

    • Western blot - Anti-CSN4 antibody (ab12322)
      Western blot - Anti-CSN4 antibody (ab12322)
      All lanes : Anti-CSN4 antibody (ab12322) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HEK293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate
      Lane 4 : TCMK-1 whole cell lysate
      Lane 5 : NIH/3T3 whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 49 kDa


      Exposure time: 3 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab12322? Please let us know so that we can cite the reference in this datasheet.

    ab12322 has been referenced in 4 publications.

    • Chung D  et al. Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis. Cell Cycle 18:1135-1153 (2019). PubMed: 31057046
    • Füzesi-Levi MG  et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . PubMed: 24421388
    • Bhansali M & Shemshedini L COP9 subunits 4 and 5 target soluble guanylyl cyclase a1 and p53 in prostate cancer cells. Mol Endocrinol 28:834-45 (2014). PubMed: 24725084
    • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-COPS4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (amniotic fluid stem cells)
    Loading amount
    5 µg
    Specification
    amniotic fluid stem cells
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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