Anti-CSN4 antibody (ab12322)
Key features and details
- Rabbit polyclonal to CSN4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CSN4 antibody
See all CSN4 primary antibodies -
Description
Rabbit polyclonal to CSN4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide within Human CSN4 aa 350 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BT78 -
Positive control
- WB: HeLa, HEK293T, Jurkat, TCMK-1, NIH/3T3
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
Target
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Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
Sequence similarities
Belongs to the CSN4 family.
Contains 1 PCI domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 422594 Chicken
- Entrez Gene: 461207 Chimpanzee
- Entrez Gene: 540223 Cow
- Entrez Gene: 51138 Human
- Entrez Gene: 26891 Mouse
- Entrez Gene: 100125960 Pig
- Entrez Gene: 360915 Rat
- Entrez Gene: 693435 Rhesus monkey
see all -
Alternative names
- CH4 antibody
- COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
- COP9 complex homolog subunit 4 antibody
see all
Images
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Western blot - Anti-CSN4 antibody (ab12322)All lanes : Anti-CSN4 antibody (ab12322) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 49 kDa
Exposure time: 3 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab12322 has been referenced in 4 publications.
- Chung D et al. Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis. Cell Cycle 18:1135-1153 (2019). PubMed: 31057046
- Füzesi-Levi MG et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . PubMed: 24421388
- Bhansali M & Shemshedini L COP9 subunits 4 and 5 target soluble guanylyl cyclase a1 and p53 in prostate cancer cells. Mol Endocrinol 28:834-45 (2014). PubMed: 24725084
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822