Anti-CSN4 antibody (ab12322)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-CSN4 antibody
See all CSN4 primary antibodies -
DescriptionRabbit polyclonal to CSN4
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to Human CSN4 (C terminal).
Database link: 51138 -
Positive control
- HEK 293T cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified using the peptide immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab12322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/5000 - 1/25000. Predicted molecular weight: 49 kDa. |
Target
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FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
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Sequence similaritiesBelongs to the CSN4 family.
Contains 1 PCI domain. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 422594 Chicken
- Entrez Gene: 461207 Chimpanzee
- Entrez Gene: 540223 Cow
- Entrez Gene: 51138 Human
- Entrez Gene: 26891 Mouse
- Entrez Gene: 100125960 Pig
- Entrez Gene: 360915 Rat
- Entrez Gene: 693435 Rhesus monkey
see all -
Alternative names
- CH4 antibody
- COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
- COP9 complex homolog subunit 4 antibody
see all
Images
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Western blot - Anti-CSN4 antibody (ab12322)Detection of Human CSN4 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN4 expression construct (T). Antibody: Affinity purified rabbit anti-CSN4 antibody BL1061 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
Detection of Human CSN4 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN4 expression construct (T).
Antibody: Affinity purified rabbit anti-CSN4 antibody BL1061 used at the indicated concentrations.
Detection: Chemiluminescence with a 10 second exposure. -
Immunocytochemistry/ Immunofluorescence - Anti-CSN4 antibody (ab12322)ICC/IF image of ab12322 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Füzesi-Levi MG et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . Read more (PubMed: 24421388) »
- Bhansali M & Shemshedini L COP9 subunits 4 and 5 target soluble guanylyl cyclase a1 and p53 in prostate cancer cells. Mol Endocrinol 28:834-45 (2014). Read more (PubMed: 24725084) »
