Western blot abreview for Anti-COPS4 antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (amniotic fluid stem cells)
Loading amount
5 µg
Specification
amniotic fluid stem cells
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking solution

Secondary antibody

Dilution
1/10000

Detection

Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 39 kDa

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Submitted Dec 15 2010

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