Overview

  • Product name

  • Description

    Rabbit polyclonal to CSN4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human CSN4 (internal sequence). The exact sequence is proprietary.
    Database link: Q9BT78

  • Positive control

    • WB: HCT 116 whole cell lysate. IHC-P: MDA-MB157 xenograft tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as COPS4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Sequence similarities

    Belongs to the CSN4 family.
    Contains 1 PCI domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CH4 antibody
    • COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
    • COP9 complex homolog subunit 4 antibody
    • COP9 complex S4 antibody
    • COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis) antibody
    • COP9 constitutive photomorphogenic homolog subunit 4 antibody
    • COP9 signalosome complex subunit 4 antibody
    • COP9 signalosome subunit 4 antibody
    • COPS4 antibody
    • CSN complex subunit 4 antibody
    • CSN4 antibody
    • CSN4 SCHPO antibody
    • CSN4_HUMAN antibody
    • DCH4 antibody
    • JAB1 containing signalosome subunit 4 antibody
    • JAB1-containing signalosome subunit 4 antibody
    • MGC94896 antibody
    • SGN4 antibody
    • Signalosome subunit 4 antibody
    see all

Images

  • Anti-CSN4 antibody (ab229115) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 46 kDa



    10% SDS-PAGE

  • Paraffin-embedded MDA-MB157 xenograft tissue stained for CSN4 with ab229115 at 1/100 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CSN4 (green) using ab229115 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229115 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229115.
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