Product nameAnti-CSN4 antibody
See all CSN4 primary antibodies
DescriptionRabbit polyclonal to CSN4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
Recombinant fragment within Human CSN4 (internal sequence). The exact sequence is proprietary.
Database link: Q9BT78
- WB: HCT 116 whole cell lysate. IHC-P: MDA-MB157 xenograft tissue. ICC/IF: HeLa cells.
This product was previously labelled as COPS4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab229115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Sequence similaritiesBelongs to the CSN4 family.
Contains 1 PCI domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CH4 antibody
- COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
- COP9 complex homolog subunit 4 antibody
Anti-CSN4 antibody (ab229115) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Paraffin-embedded MDA-MB157 xenograft tissue stained for CSN4 with ab229115 at 1/100 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CSN4 (green) using ab229115 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
ab229115 has not yet been referenced specifically in any publications.