Key features and details
- Rabbit polyclonal to CSN4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CSN4 antibody
See all CSN4 primary antibodies
DescriptionRabbit polyclonal to CSN4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
Recombinant fragment within Human CSN4 (internal sequence). The exact sequence is proprietary.
Database link: Q9BT78
- WB: HCT 116 whole cell lysate. IHC-P: MDA-MB157 xenograft tissue. ICC/IF: HeLa cells.
This product was previously labelled as COPS4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
Our Abpromise guarantee covers the use of ab229115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Sequence similaritiesBelongs to the CSN4 family.
Contains 1 PCI domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CH4 antibody
- COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
- COP9 complex homolog subunit 4 antibody
Anti-CSN4 antibody (ab229115) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Paraffin-embedded MDA-MB157 xenograft tissue stained for CSN4 with ab229115 at 1/100 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CSN4 (green) using ab229115 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229115 has not yet been referenced specifically in any publications.