Recombinant Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)
![Western blot - Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)](https://www.abcam.com/ps/products/248/ab248882/Images/ab248882-398454-anti-csn4-antibody-epr7453-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7453] to CSN4 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CSN4 antibody [EPR7453] - BSA and Azide free
See all CSN4 primary antibodies -
Description
Rabbit monoclonal [EPR7453] to CSN4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248882 is the carrier-free version of ab139688 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248882 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as COPS4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7453 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. |
Target
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Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
Sequence similarities
Belongs to the CSN4 family.
Contains 1 PCI domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51138 Human
- Entrez Gene: 26891 Mouse
- Entrez Gene: 360915 Rat
- SwissProt: Q9BT78 Human
- SwissProt: O88544 Mouse
- SwissProt: Q68FS2 Rat
- Unigene: 190384 Human
- Unigene: 957 Mouse
see all -
Alternative names
- CH4 antibody
- COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
- COP9 complex homolog subunit 4 antibody
see all
Images
-
Western blot - Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)All lanes : Anti-CSN4 antibody [EPR7453] (ab139688) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDaThis data was developed using ab139688, the same antibody clone in a different buffer formulation.
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![Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)](https://www.abcam.com/ps/products/248/ab248882/Images/ab248882-2-benefits-of-recombinant-antibodies.png)
Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab248882 has not yet been referenced specifically in any publications.
