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RecombinantRabMAb

Recombinant Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)
  • Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7453] to CSN4 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

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Overview

  • Product name

    Anti-CSN4 antibody [EPR7453] - BSA and Azide free
    See all CSN4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7453] to CSN4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248882 is the carrier-free version of ab139688 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248882 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as COPS4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7453
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Alternative Versions

    • Anti-CSN4 antibody [EPR7453] (ab139688)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
    • Sequence similarities

      Belongs to the CSN4 family.
      Contains 1 PCI domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession Q9BT78 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51138 Human
      • Entrez Gene: 26891 Mouse
      • Entrez Gene: 360915 Rat
      • SwissProt: Q9BT78 Human
      • SwissProt: O88544 Mouse
      • SwissProt: Q68FS2 Rat
      • Unigene: 190384 Human
      • Unigene: 957 Mouse
      • Unigene: 7506 Rat
      see all
    • Alternative names

      • CH4 antibody
      • COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
      • COP9 complex homolog subunit 4 antibody
      • COP9 complex S4 antibody
      • COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis) antibody
      • COP9 constitutive photomorphogenic homolog subunit 4 antibody
      • COP9 signalosome complex subunit 4 antibody
      • COP9 signalosome subunit 4 antibody
      • COPS4 antibody
      • CSN complex subunit 4 antibody
      • CSN4 antibody
      • CSN4 SCHPO antibody
      • CSN4_HUMAN antibody
      • DCH4 antibody
      • JAB1 containing signalosome subunit 4 antibody
      • JAB1-containing signalosome subunit 4 antibody
      • MGC94896 antibody
      • SGN4 antibody
      • Signalosome subunit 4 antibody
      see all

    Images

    • Western blot - Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)
      Western blot - Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)
      All lanes : Anti-CSN4 antibody [EPR7453] (ab139688) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 46 kDa



      This data was developed using ab139688, the same antibody clone in a different buffer formulation.

       

    • Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)
      Anti-CSN4 antibody [EPR7453] - BSA and Azide free (ab248882)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab248882? Please let us know so that we can cite the reference in this datasheet.

    ab248882 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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