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    csn7a-antibody-ab235400.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-CSN7A antibody (ab235400)

  • Datasheet
  • SDS
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Western blot - Anti-CSN7A antibody (ab235400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7A antibody (ab235400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7A antibody (ab235400)

Key features and details

  • Rabbit polyclonal to CSN7A
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CSN7A antibody
    See all CSN7A primary antibodies
  • Description

    Rabbit polyclonal to CSN7A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human CSN7A aa 2-275.
    Sequence:

    SAEVKVTGQNQEQFLLLAKSAKGAALATLIHQVLEAPGVYVFGELLDMPN VRELAESDFASTFRLLTVFAYGTYADYLAEARNLPPLTEAQKNKLRHLSV VTLAAKVKCIPYAVLLEALALRNVRQLEDLVIEAVYADVLRGSLDQRNQR LEVDYSIGRDIQRQDLSAIARTLQEWCVGCEVVLSGIEEQVSRANQHKEQ QLGLKQQIESEVANLKKTIKVTTAAAAAATSQDPEQHLTELREPAPGTNQ RQPSKKASKGKGLRGSAKIWSKSN


    Database link: Q9UBW8
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse brain lysate. IHC-P: Human adrenal gland and placenta tissues.
  • General notes

     This product was previously labelled as COPS7A

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • SCF Complex E3 Ligase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)
  • Related Products

    • Recombinant Human CSN7A protein (denatured) (ab180281)

Applications

Our Abpromise guarantee covers the use of ab235400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 30 kDa.
IHC-P 1/20 - 1/200.

Target

  • Function

    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Tissue specificity

    Widely expressed. Expressed at high level in brain, heart and skeletal muscle.
  • Sequence similarities

    Belongs to the CSN7/EIF3M family. CSN7 subfamily.
    Contains 1 PCI domain.
  • Post-translational
    modifications

    Phosphorylated by CK2 and PKD kinases.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q9UBW8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50813 Human
    • Entrez Gene: 26894 Mouse
    • Entrez Gene: 100173397 Orangutan
    • SwissProt: Q9UBW8 Human
    • SwissProt: Q9CZ04 Mouse
    • SwissProt: Q5R762 Orangutan
    • Unigene: 530823 Human
    • Unigene: 244536 Mouse
    • Alternative names

      • COP9 complex subunit 7a antibody
      • COP9 constitutive photomorphogenic homolog subunit 7A antibody
      • COP9 signalosome complex subunit 7a antibody
      • COP9 signalosome subunit 7A antibody
      • COPS7A antibody
      • CSN7A antibody
      • CSN7A_HUMAN antibody
      • Dermal papilla derived protein 10 antibody
      • Dermal papilla-derived protein 10 antibody
      • DERP10 antibody
      • JAB1 containing signalosome subunit 7a antibody
      • JAB1-containing signalosome subunit 7a antibody
      • SGN7a antibody
      • Signalosome subunit 7a antibody
      see all

    Images

    • Western blot - Anti-CSN7A antibody (ab235400)
      Western blot - Anti-CSN7A antibody (ab235400)
      Anti-CSN7A antibody (ab235400) at 1/1000 dilution + Mouse brain tissue lysate

      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution

      Predicted band size: 30 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7A antibody (ab235400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7A antibody (ab235400)

      Paraffin-embedded human adrenal gland tissue stained for CSN7A using ab235400 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7A antibody (ab235400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7A antibody (ab235400)

      Paraffin-embedded human placenta tissue stained for CSN7A using ab235400 at 1/100 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab235400? Please let us know so that we can cite the reference in this datasheet.

    ab235400 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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