Key features and details
- Rabbit polyclonal to CSN7b
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CSN7b antibody
See all CSN7b primary antibodies
DescriptionRabbit polyclonal to CSN7b
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Immunogen was a synthetic peptide, which represented a portion of human constitutive photomorphogenic homolog 7b encoded within exon 10.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using the peptide (immunogen) immobilized on solid support.
Our Abpromise guarantee covers the use of ab11895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 33 kDa.|
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Sequence similaritiesBelongs to the CSN7/EIF3M family. CSN7 subfamily.
Contains 1 PCI domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- COP9 Complex Homolog Subunit 7b antibody
- COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody
- COP9 Signalasome Subunit 7b antibody
RIPA lysate (50
µg for E; 25 µg for T) from HEK293T cells that were mock transfected (E) or transfected with a CSN7b construct (T). Antibody: ab11895 used at the indicated concentration. Detection: Chemiluminescence with an exposure time of 30 seconds.
RIPA lysate (50µg for E; 25µg for T) from HEK293T cells that were mock transfected (E) or transfected with a CSN7b construct (T). Antibody: ab11895 used at the indicated concentration. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab11895 has been referenced in 1 publication.
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822