For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    csn7b-antibody-epr6464-ab133548.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Intracellular Signaling Kinases
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CSN7b antibody [EPR6464] (ab133548)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
  • Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
  • Flow Cytometry (Intracellular) - Anti-CSN7b antibody [EPR6464] (ab133548)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7b antibody [EPR6464] (ab133548)
  • Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
  • OI-RD Scanning - Anti-CSN7b antibody [EPR6464] (ab133548)
  • Anti-CSN7b antibody [EPR6464] (ab133548)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6464] to CSN7b
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-PPM1A antibody [7F12] (ab135249)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-CSN7b antibody [EPR6464]
    See all CSN7b primary antibodies
  • Description

    Rabbit monoclonal [EPR6464] to CSN7b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human CSN7b aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, HAP1, Jurkat and HeLa cell lysates. IHC-P: Human kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.12 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6464
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation

Associated products

  • Alternative Versions

    • Anti-CSN7b antibody [EPR6464] - BSA and Azide free (ab248547)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human COPS7B (CSN7b) knockout HEK-293T cell line (ab266646)
  • KO cell lysates

    • Human COPS7B (CSN7b) knockout HEK-293T cell lysate (ab257895)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/200.
WB (1)
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/200.
WB
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Sequence similarities

    Belongs to the CSN7/EIF3M family. CSN7 subfamily.
    Contains 1 PCI domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q9H9Q2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64708 Human
    • Entrez Gene: 26895 Mouse
    • Entrez Gene: 363273 Rat
    • Omim: 616010 Human
    • SwissProt: Q9H9Q2 Human
    • SwissProt: Q8BV13 Mouse
    • Unigene: 335061 Human
    • Unigene: 12973 Mouse
    • Alternative names

      • COP9 Complex Homolog Subunit 7b antibody
      • COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody
      • COP9 Signalasome Subunit 7b antibody
      • COP9 signalosome complex subunit 7b antibody
      • COPS7B antibody
      • CSN7B_HUMAN antibody
      • JAB1 Containing Signalosome Subunit 7b antibody
      • JAB1-containing signalosome subunit 7b antibody
      • SGN7b antibody
      • Signalosome subunit 7b antibody
      see all

    Images

    • Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
      Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
      All lanes : Anti-CSN7b antibody [EPR6464] (ab133548) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : COPS7B knockout HEK293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HAP1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 30 kDa
      Observed band size: 32 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab133548 observed at 32 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab133548 Anti-CSN7b antibody [EPR6464] was shown to specifically react with CSN7b in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266646 (knockout cell lysate ab257895) was used.  Wild-type and CSN7b knockout samples were subjected to SDS-PAGE.  ab133548 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
      Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CSN7b knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab133548 observed at 32 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab133548 was shown to specifically react with CSN7b when CSN7b knockout samples were used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab133548 and ab18058 (loading control to vinculin) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-CSN7b antibody [EPR6464] (ab133548)
      Flow Cytometry (Intracellular) - Anti-CSN7b antibody [EPR6464] (ab133548)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CSN7b with unpurified ab133548 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7b antibody [EPR6464] (ab133548)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7b antibody [EPR6464] (ab133548)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling CSN7b with ab133548 antibody at a dilution of 1/250.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
      Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)
      All lanes : Anti-CSN7b antibody [EPR6464] (ab133548) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa

    • OI-RD Scanning - Anti-CSN7b antibody [EPR6464] (ab133548)
      OI-RD Scanning - Anti-CSN7b antibody [EPR6464] (ab133548)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CSN7b antibody [EPR6464] (ab133548)
      Anti-CSN7b antibody [EPR6464] (ab133548)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab133548? Please let us know so that we can cite the reference in this datasheet.

    ab133548 has been referenced in 2 publications.

    • Liang E  et al. COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. Neuropsychiatr Dis Treat 17:1217-1227 (2021). PubMed: 33911869
    • Liu J  et al. A genome-scale CRISPR-Cas9 screening in myeloma cells identifies regulators of immunomodulatory drug sensitivity. Leukemia N/A:N/A (2018). PubMed: 30026574

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-CSN7b antibody [EPR6464]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis-Tris gel Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.