Anti-CSN7b antibody [EPR6465] (ab124718)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-CSN7b antibody [EPR6465]
See all CSN7b primary antibodies -
DescriptionRabbit monoclonal [EPR6465] to CSN7b
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human CSN7b aa 200-300. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa, HL-60, and HT-29 cell lysates; HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR6465
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 30 kDa. | |
| IP | 1/100 - 1/500. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | 1/50 - 1/100. |
Target
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FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
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Sequence similaritiesBelongs to the CSN7/EIF3M family. CSN7 subfamily.
Contains 1 PCI domain. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 64708 Human
- Entrez Gene: 26895 Mouse
- Entrez Gene: 363273 Rat
- Omim: 616010 Human
- SwissProt: Q9H9Q2 Human
- SwissProt: Q8BV13 Mouse
- Unigene: 335061 Human
- Unigene: 12973 Mouse
see all -
Alternative names
- COP9 Complex Homolog Subunit 7b antibody
- COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody
- COP9 Signalasome Subunit 7b antibody
see all
Images
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![Flow Cytometry - Anti-CSN7b antibody [EPR6465] (ab124718)](https://www.abcam.com/ps/products/124/ab124718/Images/ab124718-275857-ab124718FC1.jpg)
Flow Cytometry - Anti-CSN7b antibody [EPR6465] (ab124718)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CSN7b with unpurified ab124718 at 1/800 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)](https://www.abcam.com/ps/products/124/ab124718/Images/ab124718-266732-ab124718KOWB.jpg)
Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CSN7b knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124718 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124718 was shown to specifically react with CSN7b when CSN7b knockout samples were used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab124718 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)](https://www.abcam.com/ps/products/124/ab124718/Images/CSN7b-Primary-antibodies-ab124718-1.jpg)
Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)All lanes : Anti-CSN7b antibody [EPR6465] (ab124718) at 1/10000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HL60 cell lysates
Lane 4 : HT-29 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CSN7b antibody [EPR6465] (ab124718)](https://www.abcam.com/ps/products/124/ab124718/Images/CSN7b-Primary-antibodies-ab124718-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CSN7b antibody [EPR6465] (ab124718)ab124718, at 1/50, staining CSN7b in HeLa cells by Immunofluorescence.
Protocols
References
This product has been referenced in:
- Gaspar L et al. The genomic landscape of human cellular circadian variation points to a novel role for the signalosome. Elife 6:N/A (2017). Read more (PubMed: 28869038) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"