Overview

  • Product name

  • Description

    Rabbit polyclonal to CSN8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CSN8 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93795)

  • Positive control

    • This antibody gave a positive signal in following human lysates: Brain tissue, HepG2 whole cell, SHSY5Y whole cell, SAOS2 whole cell.
  • General notes

     This product was previously labelled as COPS8

     

Properties

Applications

Our Abpromise guarantee covers the use of ab77300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Sequence similarities

    Belongs to the CSN8 family.
    Contains 1 PCI domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • COP9 homolog antibody
    • COP9 signalosome complex subunit 8 antibody
    • COP9 signalosome subunit 8 antibody
    • Cops8 antibody
    • CSN8 antibody
    • CSN8_HUMAN antibody
    • hCOP9 antibody
    • JAB1 containing signalosome subunit 8 antibody
    • JAB1-containing signalosome subunit 8 antibody
    • SGN8 antibody
    • Signalosome subunit 8 antibody
    see all

Images

  • All lanes : Anti-CSN8 antibody (ab77300) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa
    Additional bands at: 120 kDa, 49 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • ICC/IF image of ab77300 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

References

This product has been referenced in:

  • Crone SG  et al. microRNA-146a inhibits G protein-coupled receptor-mediated activation of NF-?B by targeting CARD10 and COPS8 in gastric cancer. Mol Cancer 11:71 (2012). WB . Read more (PubMed: 22992343) »
See 1 Publication for this product

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