Key features and details
- Rabbit polyclonal to CSN8
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CSN8 antibody
See all CSN8 primary antibodies
DescriptionRabbit polyclonal to CSN8
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
Synthetic peptide corresponding to Human CSN8 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in following human lysates: Brain tissue, HepG2 whole cell, SHSY5Y whole cell, SAOS2 whole cell.
This product was previously labelled as COPS8
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- SCF Complex E3 Ligase
Our Abpromise guarantee covers the use of ab77300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Sequence similaritiesBelongs to the CSN8 family.
Contains 1 PCI domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- COP9 homolog antibody
- COP9 signalosome complex subunit 8 antibody
- COP9 signalosome subunit 8 antibody
All lanes : Anti-CSN8 antibody (ab77300) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 120 kDa, 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
ICC/IF image of ab77300 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.
ab77300 has been referenced in 1 publication.
- Crone SG et al. microRNA-146a inhibits G protein-coupled receptor-mediated activation of NF-?B by targeting CARD10 and COPS8 in gastric cancer. Mol Cancer 11:71 (2012). WB . PubMed: 22992343