Recombinant
RabMAb

Recombinant Anti-CSN8 antibody [EPR5139] (ab124779)

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Overview

  • Product name

    Anti-CSN8 antibody [EPR5139]
    See all CSN8 primary antibodies

  • Description

    Rabbit monoclonal [EPR5139] to CSN8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CSN8 aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Fetal liver, HepG2, SH SY5Y or HeLa cell lysates IHC-P: Human brain tissue

  • General notes

     This product was previously labelled as COPS8

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

    • Sequence similarities

      Belongs to the CSN8 family.
      Contains 1 PCI domain.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • COP9 homolog antibody
      • COP9 signalosome complex subunit 8 antibody
      • COP9 signalosome subunit 8 antibody
      • Cops8 antibody
      • CSN8 antibody
      • CSN8_HUMAN antibody
      • hCOP9 antibody
      • JAB1 containing signalosome subunit 8 antibody
      • JAB1-containing signalosome subunit 8 antibody
      • SGN8 antibody
      • Signalosome subunit 8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (ab124779)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (ab124779)

      ab124779, at 1/100, staining CSN8 in formalin fixed paraffin embedded Human brain tissue using immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-CSN8 antibody [EPR5139] (ab124779)
      Western blot - Anti-CSN8 antibody [EPR5139] (ab124779)
      All lanes : Anti-CSN8 antibody [EPR5139] (ab124779) at 1/1000 dilution

      Lane 1 : Fetal liver cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH SY5Y cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 23 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Zhang H  et al. Identification of novel dipeptidyl peptidase 9 substrates by two-dimensional differential in-gel electrophoresis. FEBS J 282:3737-57 (2015). Read more (PubMed: 26175140) »
    See 1 Publication for this product

    Publishing research using ab124779? Please let us know so that we can cite the reference in this datasheet.

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