Recombinant Anti-CSN8 antibody [EPR5139] (ab124779)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (ab124779)](https://www.abcam.com/ps/products/124/ab124779/Images/ab124779-138649-anti-cops8-antibody-epr5139-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (ab124779)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5139] to CSN8
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CSN8 antibody [EPR5139]
See all CSN8 primary antibodies -
Description
Rabbit monoclonal [EPR5139] to CSN8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CSN8 aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Fetal liver, HepG2, SH SY5Y or HeLa cell lysates IHC-P: Human brain tissue
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General notes
This product was previously labelled as COPS8
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5139 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
Sequence similarities
Belongs to the CSN8 family.
Contains 1 PCI domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10920 Human
- Entrez Gene: 108679 Mouse
- Entrez Gene: 363283 Rat
- Omim: 616011 Human
- SwissProt: Q99627 Human
- SwissProt: Q8VBV7 Mouse
- SwissProt: Q6P4Z9 Rat
- Unigene: 531713 Human
see all -
Alternative names
- COP9 homolog antibody
- COP9 signalosome complex subunit 8 antibody
- COP9 signalosome subunit 8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (ab124779)
ab124779, at 1/100, staining CSN8 in formalin fixed paraffin embedded Human brain tissue using immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-CSN8 antibody [EPR5139] (ab124779)](https://www.abcam.com/ps/products/124/ab124779/Images/ab124779-138648-anti-cops8-antibody-epr5139-western-blot.jpg)
Western blot - Anti-CSN8 antibody [EPR5139] (ab124779)All lanes : Anti-CSN8 antibody [EPR5139] (ab124779) at 1/1000 dilution
Lane 1 : Fetal liver cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
![Anti-CSN8 antibody [EPR5139] (ab124779)](https://www.abcam.com/ps/products/124/ab124779/Images/ab124779-3-benefits-of-recombinant-antibodies.png)
Anti-CSN8 antibody [EPR5139] (ab124779)
Protocols
Datasheets and documents
References (1)
ab124779 has been referenced in 1 publication.
- Zhang H et al. Identification of novel dipeptidyl peptidase 9 substrates by two-dimensional differential in-gel electrophoresis. FEBS J 282:3737-57 (2015). PubMed: 26175140
