Recombinant
RabMAb

Recombinant Anti-CSN8 antibody [EPR5139] (HRP) (ab208842)

Overview

  • Product name

    Anti-CSN8 antibody [EPR5139] (HRP)
    See all CSN8 primary antibodies
  • Description

    Rabbit monoclonal [EPR5139] to CSN8 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    within Human CSN8 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99627

  • Positive control

    • WB: HeLa and SH-SY5Y whole cell and Human Fetal Liver tissue lysates.
  • General notes

     This product was previously labelled as COPS8

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Sequence similarities

    Belongs to the CSN8 family.
    Contains 1 PCI domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • COP9 homolog antibody
    • COP9 signalosome complex subunit 8 antibody
    • COP9 signalosome subunit 8 antibody
    • Cops8 antibody
    • CSN8 antibody
    • CSN8_HUMAN antibody
    • hCOP9 antibody
    • JAB1 containing signalosome subunit 8 antibody
    • JAB1-containing signalosome subunit 8 antibody
    • SGN8 antibody
    • Signalosome subunit 8 antibody
    see all

Images

  • All lanes : Anti-CSN8 antibody [EPR5139] (HRP) (ab208842) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue
    Lane 3 : SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208842 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab208842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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