Overview

  • Product name

    Anti-CSNK2A1 antibody [13B5] - N-terminal
    See all CSNK2A1 primary antibodies
  • Description

    Mouse monoclonal [13B5] to CSNK2A1 - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Human CSNK2A1 (N terminal).
    Database link: P68400

  • General notes

     This product was previously labelled as CKII alpha

     

Properties

Applications

Our Abpromise guarantee covers the use of ab181734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/2000.
WB 1/500 - 1/5000. Predicted molecular weight: 45 kDa.

Target

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CKII alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181734 observed at 44 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab181734 was shown to specifically react with CKII alpha when CKII alpha knockout samples were used. Wild-type and CKII alpha knockout samples were subjected to SDS-PAGE. ab181734 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

References

This product has been referenced in:

  • Chen M  et al. Sex-specific activation of SK current by isoproterenol facilitates action potential triangulation and arrhythmogenesis in rabbit ventricles. J Physiol 596:4299-4322 (2018). Read more (PubMed: 29917243) »
See 1 Publication for this product

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